Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus.

• Main Authors: Achmad Fuad Hafid, Chie Aoki Utsubo, Adita Ayu Permanasari, Myrna Adianti, NIDN. 0001038207, Lydia Tumewu, Aty Widyawaruyanti, NIDN. 0026046210, Sri Puji Astuti Wahyuningsih, NIDN. 0021026604, Tutik Sri Wahyuni, NIDN. 0025107704, Maria Inge Lusida, Soetjipto, Hak Hotta
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: Medknow Publications 2017
• Subjects: Q Science (General)
• Online Access: http://repository.unair.ac.id/86472/1/Bukti%20C01.%20Cover.pdf; http://repository.unair.ac.id/86472/7/Bukti%20C01%20Karya%20Ilmiah%20%28Fulltext%29.pdf; http://repository.unair.ac.id/86472/4/Bukti%20C01.%20Validasi.pdf; http://repository.unair.ac.id/86472/2/Bukti%20C01.%20Antiviral%20activity%20of%20the%20dichloromethane%20extracts%20from%20Artocarpus%20heterophyllus%20leaves%20against%20hepatitis%20C%20virus.pdf; http://repository.unair.ac.id/86472/; https://www.researchgate.net/publication/317859771_Antiviral_activity_of_the_dichloromethane_extracts_from_Artocarpus_heterophyllus_leaves_against_Hepatitis_C_Virus
• Institution: Universitas Airlangga

Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture methodusing Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCVactivities was determined by time-of-addition experiments. The effect on HCV RNAreplication and HCV accumulation in cells were analyzed by quantitative reversetranscription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A.heterophyllus exhibited strong anti HCV activity with an inhibitory concentration (IC) value of (1.5 ± 0.6) mg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC 50 50 values being (6.5 ± 0.3) mg/mL and (9.7 ± 1.1)mg/mL, respectively. A time-of-addition studies showed that DCM extractfrom A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.