Cumulative Solar UVA–UVB Exposure Increases Heat Shock Protein (HSP) 72 on Peripheral Blood Cutaneous Lymphocytes Antigen (CLA)+T Lymphocytes in Outdoor Workers

• Main Authors: Afif Nurul Hidayati, NIDN8855610016, Widodo Jatim Pudjirahardjo, NIDN. 9907147599, Saut Sahat Pohan
• Format: Article PeerReviewed
• Language: English; English; English
• Published: American Scientific Publishers 2018
• Subjects: R Medicine (General); RL Dermatology
• Online Access: http://repository.unair.ac.id/86717/1/Cumulative%20Solar%20UVA-UVB%20Exposure%20Increases%20Heat%20Shock%20Protein%20%28HSP%29.pdf; http://repository.unair.ac.id/86717/2/Cumulative%20Solar%20UVA-UVB.pdf; http://repository.unair.ac.id/86717/3/Cumulative%20Solar%20UVA-UVB%20Exposure%20Increases%20Heat%20Shock%20Protein%20%28HSP%29%2072%20on%20Peripheral%20Blood%20Cutaneous%20Lymphocytes%20Antigen%20%28CLA%29%20%2B%20T%20Lymphocytes%20in%20Outdoor%20Workers.pdf; http://repository.unair.ac.id/86717/; https://www.ingentaconnect.com/contentone/asp/asl/2018/00000024/00000009/art00055?crawler=true&mimetype=application/pdf; https://doi.org/10.1166/asl.2018.12753
• Institution: Universitas Airlangga

Solar UVA–UVB radiation is responsible for development and continuity of life in the earth, but it can endanger human health. Heat shock proteins (HSP) play an essential role in the homeostasis of the living cell when cells exposed to environmental stress. Several investigators had proved increased Hsp72 expression on keratinocytes caused by UVA–UVB exposure, but the effects in peripheral blood Cutaneous Lymphocytes Antigen (CLA)+T lymphocytes, the cells that have a significant role in skin immunity, still have not been proven. To investigate the effect of cumulative solar UVA–UVB exposure on Hsp72 expression on peripheral blood CLA+T lymphocytes in outdoor workers. This cohort study had been conducted involving 70 male subjects consisting of 37 caddies of a golf course and 33 indoor workers (one was drop out) in Surabaya, on July to September, 20–45 years old, skin phototype IV/V. Doses of solar UVA–UVB in first four weeks and eight weeks of the study were measured by Viospor® blue line II dosimeter. Hsp72 expression on the peripheral blood CLA+T lymphocytes were measured at baseline, four weeks, and eight weeks by flow cytometry. The average dose of solar UVA–UVB received over eight weeks by caddies was 12450.5±3948.8 J/m2 whereas that obtained by the indoor workers was 1794.0±1518.5 J/m2 (p = 0.0001). The Cumulative high dose of solar UVA–UVB exposure induced the increased of Hsp72 on peripheral blood CLA+T lymphocytes (p = 0.0001). The Cumulative high dose of solar UVA–UVB exposure in subjects with high outdoor activities increases Hsp72 expression on peripheral blood CLA+T lymphocytes.