PENGARUH SUPLEMENTASI ESTRADIOL 17β TERHADAP PERSENTASE MATURASI IN VITRO OOSIT SAPI
This study aimed to determine the percentage of in vitro oocyte maturation on TCM – 199 media with estradiol 17ß supplementation. Cumulus oocyte complexes (COCs) that had been selected from the dissection media were added into the maturation media. The media was using TCM-19 which were added 0,15...
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| Main Author: | |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian Indonesian Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://repository.unair.ac.id/87151/1/KKC%20KK%20KH%20235%20-%2019%20Muf%20p-Abstrak.pdf https://repository.unair.ac.id/87151/2/KKC%20KK%20KH%20235%20-%2019%20Muf%20p-Daftar%20isi.pdf https://repository.unair.ac.id/87151/3/KKC%20KK%20KH%20235%20-%2019%20Muf%20p-Daftar%20pustaka.pdf https://repository.unair.ac.id/87151/4/KKC%20KK%20KH%20235%20-%2019%20Muf%20p.pdf https://repository.unair.ac.id/87151/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian Indonesian Indonesian |
| Summary: | This study aimed to determine the percentage of in vitro oocyte maturation
on TCM – 199 media with estradiol 17ß supplementation. Cumulus oocyte
complexes (COCs) that had been selected from the dissection media were added
into the maturation media. The media was using TCM-19 which were added 0,15
IU/ml PMSG, 0,15 IU/ml HCG, FBS and estradiol 17ß. Each petridish contained
three drops of maturation media (300 μl/drops) which were coated with mineral
oil and then incubated in a 5% CO2 incubator, with maximum humidity and
temperature of 39°C. Control (P0) was oocyte maturation without
supplementation with the hormone estradiol 17ß supplementation 1μg/ml.
Treatment 1 (P1) was oocyte maturation with 1μg/ml of estradiol 17ß
supplementation. Treatment 2 (P2) was oocyte maturation with 2μg/ml of
estradiol 17ß supplementation. The calculation of mature oocyte percentage was
using aceto-orcein staining. The data analyzed using ANOVA, the result showed
that there were no significant differences in control (P0) 50.63%, in treatment 2
(P2) 54.07%, and in treatment 3 (P3) 48.60%. Based on the result, it can be
concluded that estradiol 17ß supplementation to the maturation level of cattle
oocytes showed did not effect the percentage of in vitro oocyte maturation |
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