Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone
Objective The aim of this study is to evaluate the feasibility of human umbilical cord mesenchymal stem-cell (hUCMSC) therapy in increasing osteoporotic mandibular bone density in a rat model by determining changes in alkaline phosphatase (ALP), osteocalcin, type 1 collagen, and trabecular bone area...
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id-langga.871662023-04-16T13:09:42Z https://repository.unair.ac.id/87166/ Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone Nike Hendrijantini, NIDN. 0006105908 Poedjo Hartono, NIDN. 8823800016 Muhammad Dimas Aditya Ari, NIM. 020911007 Fedik Abdul Rantam, NIDN. 0010035907 R Medicine RK Dentistry RK641-667 Prosthetic dentistry. Prosthodontics Objective The aim of this study is to evaluate the feasibility of human umbilical cord mesenchymal stem-cell (hUCMSC) therapy in increasing osteoporotic mandibular bone density in a rat model by determining changes in alkaline phosphatase (ALP), osteocalcin, type 1 collagen, and trabecular bone area after treatment. Materials and Methods This research adopted an experimental posttest-only control group design. Thirty female Wistar rats were randomly divided into six groups, namely, a control group with rats postsham surgery (T1), osteoporotic model postovariectomy rats (T2), postovariectomy rats 4 weeks after gelatin injection (T3), postovariectomy rats 8 weeks after gelatin injection (T4), postovariectomy rats 4 weeks after hUCMSC injection (T5), and postovariectomy rats 8 weeks after hUCMSC injection (T6). The rats were all sacrificed for histological and immunohistochemical examinations of ALP, osteocalcin, type 1 collagen, and trabecular bone area. Results Increased expression of ALP, type 1 collagen, and osteocalcin, as well as increased trabecular bone area, was observed in the treatment groups compared with that in the osteoporotic groups. Conclusion hUCMSCs produce significant osteogenic effects and increase osteoporotic mandibular bone density in the animal model. Increases in bone density are demonstrated by the higher levels of ALP, osteocalcin, and type 1 collagen, as well as increases in the trabecular bone area. 2019 Article PeerReviewed text en https://repository.unair.ac.id/87166/1/NIKE%207.pdf text en https://repository.unair.ac.id/87166/3/7.%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem-Cell%20Therapy%20to%20Increase%20the%20Density%20of%20Osteoporotic%20Mandibular%20Bone.pdf text id https://repository.unair.ac.id/87166/6/per%20nike%207.7.pdf text en https://repository.unair.ac.id/87166/7/T4.pdf text id https://repository.unair.ac.id/87166/8/4.pdf Nike Hendrijantini, NIDN. 0006105908 and Poedjo Hartono, NIDN. 8823800016 and Muhammad Dimas Aditya Ari, NIM. 020911007 and Fedik Abdul Rantam, NIDN. 0010035907 (2019) Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone. European Journal of Dentistry. pp. 58-63. ISSN 1305-7464 https://www.thieme-connect.com/products/ejournals/html/10.1055/s-0039-1688527 |
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R Medicine RK Dentistry RK641-667 Prosthetic dentistry. Prosthodontics Nike Hendrijantini, NIDN. 0006105908 Poedjo Hartono, NIDN. 8823800016 Muhammad Dimas Aditya Ari, NIM. 020911007 Fedik Abdul Rantam, NIDN. 0010035907 Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
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Objective The aim of this study is to evaluate the feasibility of human umbilical cord mesenchymal stem-cell (hUCMSC) therapy in increasing osteoporotic mandibular bone density in a rat model by determining changes in alkaline phosphatase (ALP), osteocalcin, type 1 collagen, and trabecular bone area after treatment. Materials and Methods This research adopted an experimental posttest-only control group design. Thirty female Wistar rats were randomly divided into six groups, namely, a control group with rats postsham surgery (T1), osteoporotic model postovariectomy rats (T2), postovariectomy rats 4 weeks after gelatin injection (T3), postovariectomy rats 8 weeks after gelatin injection (T4), postovariectomy rats 4 weeks after hUCMSC injection (T5), and postovariectomy rats 8 weeks after hUCMSC injection (T6). The rats were all sacrificed for histological and immunohistochemical examinations of ALP, osteocalcin, type 1 collagen, and trabecular bone area. Results Increased expression of ALP, type 1 collagen, and osteocalcin, as well as increased trabecular bone area, was observed in the treatment groups compared with that in the osteoporotic groups. Conclusion hUCMSCs produce significant osteogenic effects and increase osteoporotic mandibular bone density in the animal model. Increases in bone density are demonstrated by the higher levels of ALP, osteocalcin, and type 1 collagen, as well as increases in the trabecular bone area. |
format |
Article PeerReviewed |
author |
Nike Hendrijantini, NIDN. 0006105908 Poedjo Hartono, NIDN. 8823800016 Muhammad Dimas Aditya Ari, NIM. 020911007 Fedik Abdul Rantam, NIDN. 0010035907 |
author_facet |
Nike Hendrijantini, NIDN. 0006105908 Poedjo Hartono, NIDN. 8823800016 Muhammad Dimas Aditya Ari, NIM. 020911007 Fedik Abdul Rantam, NIDN. 0010035907 |
author_sort |
Nike Hendrijantini, NIDN. 0006105908 |
title |
Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
title_short |
Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
title_full |
Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
title_fullStr |
Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
title_full_unstemmed |
Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone |
title_sort |
human umbilical cord mesenchymal stem-cell therapy to increase the density of osteoporotic mandibular bone |
publishDate |
2019 |
url |
https://repository.unair.ac.id/87166/1/NIKE%207.pdf https://repository.unair.ac.id/87166/3/7.%20Human%20Umbilical%20Cord%20Mesenchymal%20Stem-Cell%20Therapy%20to%20Increase%20the%20Density%20of%20Osteoporotic%20Mandibular%20Bone.pdf https://repository.unair.ac.id/87166/6/per%20nike%207.7.pdf https://repository.unair.ac.id/87166/7/T4.pdf https://repository.unair.ac.id/87166/8/4.pdf https://repository.unair.ac.id/87166/ https://www.thieme-connect.com/products/ejournals/html/10.1055/s-0039-1688527 |
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1764208932571578368 |