DETERMINATION OF RETENTION TIME OF MEDROXY PROGESTERONE ACETATE UNDER STORAGE TIME USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

• Main Author: ASRI KARUNIAWATI, 061511133093
• Format: Theses and Dissertations NonPeerReviewed
• Language: Indonesian; Indonesian; Indonesian; Indonesian
• Published: 2019
• Subjects: Including veterinary genetics, ethology, anatomy, physiology, embryology, pathology
• Online Access: http://repository.unair.ac.id/87230/1/KKC%20KK%20KH%20221%20-%2019%20Kar%20d-Abstrak.pdf; http://repository.unair.ac.id/87230/2/KKC%20KK%20KH%20221%20-%2019%20Kar%20d-Abstrak.pdf; http://repository.unair.ac.id/87230/3/KKC%20KK%20KH%20221%20-%2019%20Kar%20d-Daftar%20pustaka.pdf; http://repository.unair.ac.id/87230/4/KKC%20KK%20KH%20221%20-%2019%20Kar%20d.pdf; http://repository.unair.ac.id/87230/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

The aim of this research was to determine the retention time of chromatogram Medroxy Progesterone Acetate (MPA) dissolved in a solvent named Mobile Phase Eluent (MPE) after being stored for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). This research used posttest-only control group design with three treatments and six replications. The three treatments were 6, 8 and 12 hours storage time. Data obtained were analyzed by One-Way Anova. The result showed retention time of MPA at 6 hours (T1) and 8 hours (T2) storage time were stable but were not for 12 hours (T3) storage time appeared irrelevant peaks indicates the substance where was starting to break down. Based on those result, it could be concluded that Medroxy Progesterone Acetate in Mobile Phase Eluent began to show instability at 12 hours of storage time.