Antimicrobial Resistance Detection of Citrobacterfreundii Isolated From Rectal SWAB in East Java

• Main Authors: Mustofa Helmi Effendi, -, Intan Permatasari Hermawan, -, Wiwiek Tyasningsih, -, Suwarno, -, Intan Galuh Bintari, -, Risi Cicilia, -, Rinda Dewi Safitri, -
• Format: Article PeerReviewed
• Language: English; English; English; English; English
• Published: National Taiwan Normal University 2017
• Subjects: SF600-1100 Veterinary medicine; SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: https://repository.unair.ac.id/87461/6/Bukti%20C%2005.%20Antimicrobial%20Resistence%20Setection%20.....pdf; https://repository.unair.ac.id/87461/8/Peer%20review%20C%2005.pdf; https://repository.unair.ac.id/87461/9/Karil%20C%2005.%20ANTIMICROBIAL%20RESISTANCE%20DETECTION%20OF%20......pdf; https://repository.unair.ac.id/87461/19/C17_Peer_Review_Wiwiek_Tyasningsih.pdf; https://repository.unair.ac.id/87461/16/Turn%20It%20In%20Antimicrobial%20Resistance%20Detection%20of%20Citrobacterfreundii%20an%20Wiwiek%20Tyasningsih.pdf; https://repository.unair.ac.id/87461/; http://www.journalijdr.com
• Institution: Universitas Airlangga

The aim of this study was to detect blaTEMgene which the agent of antibiotic resistance in Citrobacterfreundii isolated from rectal swab in East Java. A total of 275 rectal swab samples were collected from 12 district of East Java. Then, samples were isolated with selective media and biochemical tests. Antibiotic Resistance test was using the disc diffusion against amoxycillin, amoxycillin-clavulanic acid, ampicillin-sulbactam, cefotaxime, ceftazidime, sulfametaxole-trimethoprim, and tetracycline and then detection of bla TEM gene used PCR. The number of Citrobacterfreundii was 7 (2,55 %). Antimicrobial resistance showed 2 broilers and 1 dairy cattle from Surabaya resistant to ampicillin-sulbactam and tetracycline, 4 milkfish from Sidoarjo resistant to amoxicillin and ampicillin-sulbactam. All seven samples were positive blaTEM gene with 445 bp size used PCR. Key Words:Antimicrobial resistance,Citrobacterfreundii, Rectal swab,blaTEMgene, ESBL