Production and Assay of Cellulolytic Enzyme Activity of Enterobacter Cloacae WPL 214 Isolated from Bovine Rumen Fluid Waste of Surabaya Abbatoir, Indonesia
Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia. Materials and M...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article PeerReviewed |
| Language: | English English English |
| Published: |
Veterinary World
2015
|
| Subjects: | |
| Online Access: | http://repository.unair.ac.id/87965/1/Bukti%20C%2003%20Production%20and%20Assay%20....pdf http://repository.unair.ac.id/87965/2/Peer%20Review%20Bukti%20C03.pdf http://repository.unair.ac.id/87965/8/Bukti%20C%2003.%20Production%20and%20assay%20of%20cellulolytic%20enzyme%20...._compressed.pdf http://repository.unair.ac.id/87965/ http://www.veterinaryworld.org/Vol.8/March-2015/19.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universitas Airlangga |
| Language: | English English English |
| Summary: | Aim: This study aims to produce and assay cellulolytic enzyme activity (endo-(1,4)-β-D-glucanase, exo-(1,4)-β-Dglucanase, and β-glucosidase, at optimum temperature and optimum pH) of Enterobacter cloacae WPL 214 isolated from bovine rumen fluid waste of Surabaya Abbatoir, Indonesia.
Materials and Methods: To produce enzyme from a single colony of E. cloacae WPL 214, 98 × 1010 CFU/ml of isolates was put into 20 ml of liquid medium and incubated in a shaker incubator for 16 h at 35°C in accordance with growth time and optimum temperature of E. cloacae WPL 214. Further on, culture was centrifuged at 6000 rpm at 4°C for 15 min. Pellet was discarded while supernatant containing cellulose enzyme activity was withdrawn to assay endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase, and β-glucosidase.
Results: Cellulase enzyme of E. cloacae WPL 214 isolates had endoglucanase activity of 0.09 U/ml, exoglucanase of 0.13 U/ml, and cellobiase of 0.10 U/ml at optimum temperature 35°C and optimum pH 5.
Conclusion: E. cloacae WPL 214 isolated from bovine rumen fluid waste produced cellulose enzyme with activity as cellulolytic enzyme of endo-(1,4)-β-D-glucanase, exo-(1,4)-β-D-glucanase and β-glucosidase.
Keywords: endo-(1,4)-β -D-glucanase, exo-(1,4)-β-D-glucanase, β-glucosidase., Enterobacter cloacae WPL 214. |
|---|