KARAKTERISASI MOLEKULER DAN MAPPING EPITOP PROTEIN MEMBRAN LUAR Aeromonas hydrophilla ISOLAT LOKAL SEBAGAI KANDIDAT VAKSIN AEROMONIASIS
Aeromoniasis disease or known as Ulcer Diseases caused by Aeromonas hydrophila which in fish or shrimp often occurs in some embankment or in the Aquarium can cause sign patches – patches of bleeding can even cause economic loss and death if not promptly treated with treatment that accompanied the...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | Indonesian Indonesian Indonesian Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/88028/1/Dis.%20SV.%2002-19%20Yul%20k%20ABSTRAK.pdf http://repository.unair.ac.id/88028/2/Dis.%20SV.%2002-19%20Yul%20k%20DAFTAR%20ISI.pdf http://repository.unair.ac.id/88028/3/Dis.%20SV.%2002-19%20Yul%20k%20DAFTAR%20PUSTAKA.pdf http://repository.unair.ac.id/88028/4/Dis.%20SV.%2002-19%20Yul%20k.pdf http://repository.unair.ac.id/88028/ http://lib.unair.ac.id |
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| Institution: | Universitas Airlangga |
| Language: | Indonesian Indonesian Indonesian Indonesian |
| Summary: | Aeromoniasis disease or known as Ulcer Diseases caused by Aeromonas hydrophila
which in fish or shrimp often occurs in some embankment or in the Aquarium can
cause sign patches – patches of bleeding can even cause economic loss and death if
not promptly treated with treatment that accompanied the good sanitation. This disease
affects many farmed freshwater fish in East Java. Previous research has managed to
do the characterization of antigenic protein originating from the OMP (outer
membrane protein) Aeromonas hydrophilla. Because it is felt necessary to do DNA
sequensing encoder protein in question.
To achieve these goals then do some exploratory research laboratoric methods,
namely: identification of the bacterium Aeromonas hydrophilla fish from suspect
infection by PCR, gel electrophoresis and Sequencing of gene fragments of the agarosa
OMP A. hydrophilla isolates of East Java.
The results obtained from this research is agarosa gel electrophoresis in DNA
chromosome Aeromonas hydrophila bacteria cells is carried out at a temperature of
annealing 54oC position 400 bp and the results of the sequencing primer that is used
produces a sequence of nucleotides is around 355 nucleotides. |
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