THE EFFECT OF ALPHA-TOCOPHEROL SUPPLEMENTATION IN TRIS-EGG-YOLK DILUENT ON SAPUDI RAM SPERMATOZOA MOTILITY, VIABILITY, AND PLASMA MEMBRANE INTEGRITYAFTER 24 HOURS COOLING AT 50C
The process of semen cryopreservation could cause an abrupt explosion of reactive oxygen species production. This condition shows results in spermatozoa damage and quality decrease. This study was conducted to analyze the maintaining effect of alpha-tocopherol supplementation in tris egg yolk dil...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/89234/1/abstrak.pdf http://repository.unair.ac.id/89234/2/daftar%20isi.pdf http://repository.unair.ac.id/89234/3/daftar%20pustaka.pdf http://repository.unair.ac.id/89234/4/full%20text.pdf http://repository.unair.ac.id/89234/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English English |
| Summary: | The process of semen cryopreservation could cause an abrupt explosion of
reactive oxygen species production. This condition shows results in spermatozoa
damage and quality decrease. This study was conducted to analyze the maintaining
effect of alpha-tocopherol supplementation in tris egg yolk diluent on spermatozoa
motility, viability, and plasma membrane integrity after 24 hours of cooling at 50C.
The samples were fresh semen which collected from Sapudi rams. It was divided into
four different treatment groups. The control treatment or T0 contained semen sample
+ tris egg yolk diluent, and the treatment 1, 2 and 3 or T1, T2, and T3 contained
semen sample + tris egg yolk diluent + alpha-tocopherol. The concentration of alphatocopherol
for T1, T2, and T3 were based on the volume of diluent, in the sequence
were 0.5 mM, 1 mM, and 2 mM. The least decline of spermatozoa motility, viability,
and plasma membrane integrity was found in T2, which was significantly different to
T0 (p<0.05). However, T2 did not show a significant difference in the result of
spermatozoa motility, viability, and plasma membrane integrity (p>0.05). It can be
concluded that alpha-tocopherol at the concentration of 1 mM can be an efficient
antioxidant supplement in tris egg yolk diluent for ram semen. |
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