Pengaruh Suplementasi Insulin Transferrin Selenium (ITS) pada Media Maturasi TCM-199 terhadap Diameter Oosit Sapi

• Main Authors: Reza Mahendra Yudha Permana, Widjiati, Budi Utomo, Tjuk Imam Restiadi
• Format: Article PeerReviewed
• Language: Indonesian; Indonesian
• Published: Fakultas Kedokteran Hewan Universitas Airlangga 2015
• Subjects: SF191-275 Cattle; SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: http://repository.unair.ac.id/90753/2/Bukti%20C%2020%20Pengaruh%20Suplementasi%20%20Insulin%20Transferrin%20.....pdf; http://repository.unair.ac.id/90753/1/Peer%20Review%20Bukti%20C20.pdf; http://repository.unair.ac.id/90753/
• Institution: Universitas Airlangga

Insulin Transferrin Selenium (ITS) has been used in vitro maturation system to support in vitro maturation of oocyte. The study aims was to determine role of Insulin Transferrin Selenium to bovine oocyte were matured in vitro against improved oocyte diameter. Maturation process used TCM-199 medium which added by FSH 100 IU/ml, LH 100 IU/ml, BSA 355 and ITS (Insulin Transferrin Selenium). ITS dose which used in culture medium are different for each treated group. The treated groups are Pp (0 ug/ml), P1 (10 ug/ml), Ps (15 ug/ml), and P3 (20 ug/ml). Oocytes were incubated in CO) incubator with CO, 5Y9, Humidity 95Y6 and 38.5"C due 22 hours. The result showed that ITS supplementation in maturation medium has no significantly difference (p20,05) for ali treated groups (Po. Pi, P5, and P3) of oocyte diameter improvement. Conclusion of ITS supplementation in maturation medium TCM-199 can't increase oocyte diameter. Keywords: oocyte, in vitro maturation, Insulin Transferrin Selenium, diameter