Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor

Background: The data about the association between Helicobacter pylori putative virulence factors; iceA and jhp0562/β-(1,3)galT with clinical outcomes are still controversial. We identified and analyzed two putative H. pylori virulence factors in Nepalese strains. Methods: The iceA and jhp0562/β-(1,...

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Main Authors: Rabi Prakash Sharma, Muhammad Miftahussurur, Pradeep Krishna Shrestha, Phawinee Subsomwong, Tomohisa Uchida, Yoshio Yamaoka
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Language:English
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Published: Asian Pacific Organization for Cancer Prevention 2017
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Online Access:http://repository.unair.ac.id/90843/1/2%20Nepalese%20Helicobacter%20pylori%20Genotypes%20Re%EF%AC%82ects%20a%20Geographical%20Diversity%20than%20a%20True%20Virulence%20Factor.pdf
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spelling id-langga.908432019-12-06T06:54:50Z http://repository.unair.ac.id/90843/ Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor Rabi Prakash Sharma Muhammad Miftahussurur Pradeep Krishna Shrestha Phawinee Subsomwong Tomohisa Uchida Yoshio Yamaoka R Medicine (General) RC Internal medicine Background: The data about the association between Helicobacter pylori putative virulence factors; iceA and jhp0562/β-(1,3)galT with clinical outcomes are still controversial. We identified and analyzed two putative H. pylori virulence factors in Nepalese strains. Methods: The iceA and jhp0562/β-(1,3)galT allelic types were determined by polymerase chain reaction amplification. Histological analysis were classified according to the updated Sydney system and the Operative Link on Gastritis Assessment (OLGA) system. Results: Among 49 strains, iceA1 negative/iceA2 positive (iceA2-positive) was predominant type (57.1%, 28/49) and 20 (40.8%) were iceA1 positive/iceA2 negative. The remaining one (2.0%) was positive for both iceA1 and iceA2 (iceA1/iceA2-mixed). Patients infected with iceA1-positive strains tended to be higher OLGA score than iceA2-positive strains [1.45 [1] vs. 0.07 [0.5], P = 0.09, respectively). The jhp0562 negative/β-(1,3)galT positive was predominant type (25/51, 49.0%), followed by double positive for jhp0562/β-(1,3)galT (15/51, 29.4%) and jhp0562 positive/β-(1,3)galT negative (11/51, 21.6%). Activity in the corpus was significantly higher in jhp0562 negative/β-(1,3)galT positive than double positive of jhp0562/β-(1,3)galT positive [mean (median); 1.24 (1) vs. 0.73 (1), P = 0.03]. There was association between iceA and subtype of vacA signal region (e.g., s1a, s1b or s1c) and combination subtypes of signal and middle regions (e.g., s1a-m1c) (P = 0.02, r = 0.29; and P = 0.002, r = 0.42, respectively). In addition, jhp0562/β-(1,3)galT genotypes associated with cagA pre-EPIYA type (e.g., 6 bp-, 18 bp-, or no deletion-type) (P = 0.047, r = 0.15). Conclusion: The inconsistency results of the association between iceA, jhp0562/β-(1,3)galT and histological scores suggesting that these genes may associate with genetic heterogeneity rather than as a true virulence factor. Asian Pacific Organization for Cancer Prevention 2017-10 Article PeerReviewed text en http://repository.unair.ac.id/90843/1/2%20Nepalese%20Helicobacter%20pylori%20Genotypes%20Re%EF%AC%82ects%20a%20Geographical%20Diversity%20than%20a%20True%20Virulence%20Factor.pdf text en http://repository.unair.ac.id/90843/2/2.%20Nepalese%20Helicobacter.pdf text en http://repository.unair.ac.id/90843/5/Nepalease.pdf Rabi Prakash Sharma and Muhammad Miftahussurur and Pradeep Krishna Shrestha and Phawinee Subsomwong and Tomohisa Uchida and Yoshio Yamaoka (2017) Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor. Asian Pacific Journal of Cancer Prevention, 18 (10). pp. 2637-2641. ISSN 2476-762X http://journal.waocp.org/article_51150.html 10.22034/APJCP.2017.18.10.2637
institution Universitas Airlangga
building Universitas Airlangga Library
country Indonesia
collection UNAIR Repository
language English
English
English
topic R Medicine (General)
RC Internal medicine
spellingShingle R Medicine (General)
RC Internal medicine
Rabi Prakash Sharma
Muhammad Miftahussurur
Pradeep Krishna Shrestha
Phawinee Subsomwong
Tomohisa Uchida
Yoshio Yamaoka
Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
description Background: The data about the association between Helicobacter pylori putative virulence factors; iceA and jhp0562/β-(1,3)galT with clinical outcomes are still controversial. We identified and analyzed two putative H. pylori virulence factors in Nepalese strains. Methods: The iceA and jhp0562/β-(1,3)galT allelic types were determined by polymerase chain reaction amplification. Histological analysis were classified according to the updated Sydney system and the Operative Link on Gastritis Assessment (OLGA) system. Results: Among 49 strains, iceA1 negative/iceA2 positive (iceA2-positive) was predominant type (57.1%, 28/49) and 20 (40.8%) were iceA1 positive/iceA2 negative. The remaining one (2.0%) was positive for both iceA1 and iceA2 (iceA1/iceA2-mixed). Patients infected with iceA1-positive strains tended to be higher OLGA score than iceA2-positive strains [1.45 [1] vs. 0.07 [0.5], P = 0.09, respectively). The jhp0562 negative/β-(1,3)galT positive was predominant type (25/51, 49.0%), followed by double positive for jhp0562/β-(1,3)galT (15/51, 29.4%) and jhp0562 positive/β-(1,3)galT negative (11/51, 21.6%). Activity in the corpus was significantly higher in jhp0562 negative/β-(1,3)galT positive than double positive of jhp0562/β-(1,3)galT positive [mean (median); 1.24 (1) vs. 0.73 (1), P = 0.03]. There was association between iceA and subtype of vacA signal region (e.g., s1a, s1b or s1c) and combination subtypes of signal and middle regions (e.g., s1a-m1c) (P = 0.02, r = 0.29; and P = 0.002, r = 0.42, respectively). In addition, jhp0562/β-(1,3)galT genotypes associated with cagA pre-EPIYA type (e.g., 6 bp-, 18 bp-, or no deletion-type) (P = 0.047, r = 0.15). Conclusion: The inconsistency results of the association between iceA, jhp0562/β-(1,3)galT and histological scores suggesting that these genes may associate with genetic heterogeneity rather than as a true virulence factor.
format Article
PeerReviewed
author Rabi Prakash Sharma
Muhammad Miftahussurur
Pradeep Krishna Shrestha
Phawinee Subsomwong
Tomohisa Uchida
Yoshio Yamaoka
author_facet Rabi Prakash Sharma
Muhammad Miftahussurur
Pradeep Krishna Shrestha
Phawinee Subsomwong
Tomohisa Uchida
Yoshio Yamaoka
author_sort Rabi Prakash Sharma
title Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
title_short Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
title_full Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
title_fullStr Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
title_full_unstemmed Nepalese Helicobacter pylori Genotypes Reflects a Geographical Diversity than a True Virulence Factor
title_sort nepalese helicobacter pylori genotypes reflects a geographical diversity than a true virulence factor
publisher Asian Pacific Organization for Cancer Prevention
publishDate 2017
url http://repository.unair.ac.id/90843/1/2%20Nepalese%20Helicobacter%20pylori%20Genotypes%20Re%EF%AC%82ects%20a%20Geographical%20Diversity%20than%20a%20True%20Virulence%20Factor.pdf
http://repository.unair.ac.id/90843/2/2.%20Nepalese%20Helicobacter.pdf
http://repository.unair.ac.id/90843/5/Nepalease.pdf
http://repository.unair.ac.id/90843/
http://journal.waocp.org/article_51150.html
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