VALIDASI METODE KLTKT-DENSITOMETRI UNTUK PENETAPAN KADAR CAMPURAN CEMARAN DIMETIL FTALAT DAN DI-2-ETILHEKSIL FTALAT PADA PRODUK SABUN MANDI CAIR
Dimethyl phthalate (DMP) and Di(2-ethylhexyl) phthalate (DEHP) are found in many of cosmetic products such as liquid body soaps. Moreover, these compound can cause several toxicological effect as an Endocrine Disrupting Chemical (EDCs) and also have toxicological effect in both male and female r...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/90879/1/FF.KF.26-19%20Dan%20v%20abstrak.pdf http://repository.unair.ac.id/90879/2/FF.KF.26-19%20Dan%20v%20daftar%20isi.pdf http://repository.unair.ac.id/90879/3/FF.KF.26-19%20Dan%20v%20daftar%20pustaka.pdf http://repository.unair.ac.id/90879/4/FF.KF.26-19%20Dan%20v.pdf http://repository.unair.ac.id/90879/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English Indonesian |
| Summary: | Dimethyl phthalate (DMP) and Di(2-ethylhexyl) phthalate (DEHP)
are found in many of cosmetic products such as liquid body soaps.
Moreover, these compound can cause several toxicological effect as an
Endocrine Disrupting Chemical (EDCs) and also have toxicological effect
in both male and female reproductions. This study aims to validate HPTLCDensitometric
Method that have to meet the requirements of validation
method which is selectivity, linearity, limit of detection, limit of
quantification, precision and accuracy. This analysis used TLC Scanner
Camag 4 at wavelength 280 nm. Silica Gel 60F 254 used as the stationary
phase and n-hexane : ethyl acetate (8:2) as the mobile phase. The result
showed that this method was selectively separate DMP and DEHP from
other components, shown by resolution (Rs) ≥ 1.5. And also there was a
good correlation between concentration and area shown from the r value of
DMP 0.9985 and DEHP 0.9980. Limit of detection of DMP is 0.74 ppm
and DEHP is 4.05 ppm. Limit of quantification of DMP is 2.46 ppm and
DEHP is 13.51 ppm. Percentage of recovery for accuracy of DMP is (99.39
± 2.03)% and for DEHP is (97.93 ± 1.30)%. And the relative standard
deviation of DMP is 2.19% and for DEHP is 1.57%. As result, this method
can be used to determine DMP and DEHP in liquid soaps that spread at the
local market in Indonesia. |
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