PENAPISAN DAN IDENTIFIKASI BAKTERI LAUT PENGHASIL ANTIBAKTERI SIMBIOSIS Halichondria panicea DARI PANTAI CABBIYA MADURA DENGAN ANALISIS GEN 16S rRNA
One of the antibacterial sources is obtained from bacteria that symbiosis with sponges. The purpose of this study is to isolate bacteria that are symbiotic with sponges from Cabbiya Madura Coast and have a large antibacterial effect. The sponge sample was identified as Halichondria panicea. Anti...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/90904/1/FF.KF.36-19%20Bua%20p%20abstrak.pdf http://repository.unair.ac.id/90904/2/FF.KF.36-19%20Bua%20p%20daftar%20isi.pdf http://repository.unair.ac.id/90904/3/FF.KF.36-19%20Bua%20p%20daftar%20pustaka.pdf http://repository.unair.ac.id/90904/4/FF.KF.36-19%20Bua%20p.pdf http://repository.unair.ac.id/90904/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English Indonesian |
| Summary: | One of the antibacterial sources is obtained from bacteria that
symbiosis with sponges. The purpose of this study is to isolate bacteria that
are symbiotic with sponges from Cabbiya Madura Coast and have a large
antibacterial effect. The sponge sample was identified as Halichondria
panicea. Antibacterial activity was tested by initial screening using the
well-diffusion method from isolation media. Six isolates continued with
activity testing, but only two isolates produced inhibitory zones (BSM-D
and BSM-F). The inhibitory zone from BSM-D and BSM-F isolates are
12.47±0.45mm and 20.18±1.10mm respectively. So, BSM-F isolate has the
greatest antibacterial activity. BSM-F isolate inoculated on various kinds of
medium. Further tests using agar-plug diffusion method against pathogenic
bacteria that are Staphylococcus aureus ATCC 25923, Escherichia coli
ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Bacillus subtilis
FNCC 0059 so that bacterial isolates were obtained with the greatest
antibacterial activity. BSM-F that inoculated on Starch Casein Agar (SCA)
medium has the largest antibacterial activity against those pathogenic
bacteria. The identification of bacteria was carried out by Gram-staining,
biochemistry, and 16S rRNA gene analysis. BSM-F bacteria were then
characterized and identified microscopically, macroscopically,
biochemically, and 16S rRNA genes. The identification results showed that
BSM-F was a Gram-positive bacillus bacteria and based on the
phylogenetic tree shows that BSM-F had the closest kinship with Bacillus
tequilensis with a similarity of 98.72%. |
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