VALIDASI METODE KROMATOGRAFI CAIR KINERJA TINGGI UNTUK PENETAPAN KADAR KLORFENIRAMIN MALEAT DALAM TABLET YANG MENGANDUNG TARTRAZIN
ination of chlorpheniramine maleate in tablets containing tartrazine has been optimized and validated. The optimum condition of the method was achieved on μbondapak C-18 (10 μm, 300 x 3.9 mm) with the mobile phase consists of 0.05 M phosphate buffer adjusted with 85% orthophosphoric acid to pH 4...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/90945/1/FF.KF.44-19%20Yul%20v%20abstrak.pdf http://repository.unair.ac.id/90945/2/FF.KF.44-19%20Yul%20v%20daftar%20isi.pdf http://repository.unair.ac.id/90945/3/FF.KF.44-19%20Yul%20v%20daftar%20pustaka.pdf http://repository.unair.ac.id/90945/4/FF.KF.44-19%20Yul%20v.pdf http://repository.unair.ac.id/90945/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English Indonesian |
| Summary: | ination of chlorpheniramine maleate in tablets containing tartrazine
has been optimized and validated. The optimum condition of the method was
achieved on μbondapak C-18 (10 μm, 300 x 3.9 mm) with the mobile phase
consists of 0.05 M phosphate buffer adjusted with 85% orthophosphoric acid
to pH 4 and methanol in the ratio of 70:30 v/v in isocratic system at the flow
rate of 1.0 mL/minute. Detection was carried out at the λ of 264 nm and the
column temperature was set at 40 °C. The validation parameters, including
specificity, linearity, accuracy, and precision were evaluated. A specificity
test showed that no other peaks detected at the similar retention time of
chlorpheniramine maleate. The method shows good linearity at
concentrations range of 10 ppm, 20ppm, 40 ppm, 60 ppm, 80 ppm with the
regression equation of y = 12526x + 19662 correlation coefficients of r>
0.999 and Vxo of <5% namely r = 0.99951 and Vxo=2, 45%. The accuracy
of the method was found to be 99.62% with precision (RSD) less than 2%.
Based on one way ANOVA analysis, a HPLC method and standard method
from Indonesian Pharmacopeia have no significant differences. Which means
that alternative methods can be used for the determination of
chlorpheniramine maleate other than the standard method. The proposed
method was proved to be valid and could be used for the determination of
chlorpheniramine maleate in tablets containing tartrazine. |
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