PURIFIKASI DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDER FRAKSI-3 EKSTRAK ETIL ASETAT JAMUR ENDOFIT Aspergillus salwaensis strain DTO297C1 DIISOLASI DARI Chromolaena odorata
Endophytic microbes that live in plant tissues without endangering their hosts. Endophytets produce some substances that have biological activity. Aspergillus salwaensi DTO297C1 is one of the endophytic fungi isoted from ethyl acetate extract isolated from Chromolaena odorata was known to have a...
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| Format: | Theses and Dissertations NonPeerReviewed |
| Language: | English English English Indonesian |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/90947/1/FF.KF.45-19%20Pus%20p%20abstrak.pdf http://repository.unair.ac.id/90947/2/FF.KF.45-19%20Pus%20p%20daftar%20isi.pdf http://repository.unair.ac.id/90947/3/FF.KF.45-19%20Pus%20p%20daftar%20pustaka.pdf http://repository.unair.ac.id/90947/4/FF.KF.45-19%20Pus%20p.pdf http://repository.unair.ac.id/90947/ |
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| Institution: | Universitas Airlangga |
| Language: | English English English Indonesian |
| Summary: | Endophytic microbes that live in plant tissues without endangering
their hosts. Endophytets produce some substances that have biological
activity. Aspergillus salwaensi DTO297C1 is one of the endophytic fungi
isoted from ethyl acetate extract isolated from Chromolaena odorata was
known to have antimicrobial and antifungal activity against S.aureus,
B.subtilis, E.coli and Candida albicans fungi. The 3 fraction of the extract is
known to have antibacterial activity. The objective of this study was to purify
and identification metabolite from 3 fraction. Purification using column
chromatography with silica gel 60 for column chromatography and n-hexane
: etil acetate : methanol 5% gradient eluent. was then carried out and 31 subfractions
were obtained. The analysis was continued with GC-FID and GCMS.
16 sub-fractions were analyzed, there were 21 compounds that could be
identified based on the dominant peak, fragmentation pattern and suitability
of compound fragmentation with the literature. |
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