Hepatitis B Virus X Gene Mutation With Predominance A1762T+G1764A Double Mutation on Chronic Liver Disease Patients in Surabaya, Indonesia

• Main Authors: Citrawati Dyah Kencono Wungu, Mochamad Amin, S. Eriaty N. Ruslan, Priyo Budi Purwono, Ulfa Kholili, Gwenny Ichsan Prabowo, Ummi Maimunah, Poernomo Boedi Setiawan, Maria Inge Lusida, Soetjipto, Retno Handajani
• Format: Article PeerReviewed
• Language: English; English; English
• Published: Malaysian Society for Biochemistry & Molecular Biology 2019
• Subjects: R Medicine (General); RA Public aspects of medicine
• Online Access: http://repository.unair.ac.id/91412/2/Hepatitis%20B%20Virus%20X%20Gene%20Mutation%20With%20Predominance%20A1762T%2BG1764A%20Double%20Mutation%20on%20Chronic%20Liver%20Disease%20Patients%20In%20Surabaya%2C%20Indonesia.pdf; http://repository.unair.ac.id/91412/3/Hepatitis%20B%20Virus%20X%20Gene%20Mutation%20With%20Predominance%20A1762T%2BG1764A%20Double%20Mutation%20on%20Chronic%20Liver%20Disease%20Patients%20In%20Surabaya%2C%20Indonesia.pdf; http://repository.unair.ac.id/91412/7/Hepatitis%20B%20Virus.pdf; http://repository.unair.ac.id/91412/; https://msbmb2010.wixsite.com/mjbmb/copy-of-home-5
• Institution: Universitas Airlangga

Background: HBV infection is a major problem worldwide, especially in developing countries including Indonesia. Mutations in the HBV gene X are commonly found in patients with CLD, especially in cirrhosis and hepatocellular carcinoma. Mutations in the X gene can cause loss of stability, increased transactivation function, and decreased anti-apoptotic ability of HBx protein. Aim: The aim of this study was to detect HBV X gene mutations in CLD patients in Surabaya. Methods: This was a cross sectional research taking samples at Dr. Soetomo General Hospital, Surabaya, Indonesia. This study used nested PCR by targeting HBV X gene. Samples showing positive HBV DNA PCR results were followed by sequencing and X gene mutation analysis by comparing sequencing results with reference strains. Results: In this study, 30 samples of CLD patients with positive HBsAg in Dr. Soetomo Surabaya were obtained. From the results of the multiple alignments, 12/30 samples (40%) had mutations on HBV X region which overlapped with Core Promoter region. There were 3 types of substitution mutations on HBV X gene (C1632T, T1753A/C/G, A1762T, and G1764A) with the dominant mutation types were A1762T and G1764A mutations, in which both mutations were found together as double mutation. Conclusion: X gene mutations were found in 40% CLD patients in Surabaya with the dominant mutation was in the form of double mutation A1762T and G1764A in 30% CLD patients in this study. The mutation was found mostly in advance stage of CLD.