Karakterisasi Pola Gen 16s Rrna Streptomyces sp. Penghasil Antibiotik Isolat Tanah Ekosistem Mangrove di Jawa Timur

• Main Authors: Wiwin Retnowati, Kuntaman, Isnaeni
• Format: Other NonPeerReviewed
• Language: Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian; Indonesian
• Published: Universitas Airlangga 2007
• Subjects: QK1-989 Botany; QR1-502 Microbiology
• Online Access: http://repository.unair.ac.id/94111/1/1.%20JUDUL.pdf; http://repository.unair.ac.id/94111/2/2.%20ABSTRAK.pdf; http://repository.unair.ac.id/94111/3/3.%20DAFTAR%20ISI.pdf; http://repository.unair.ac.id/94111/4/4.%20BAB%20I.pdf; http://repository.unair.ac.id/94111/5/5.%20BAB%20II.pdf; http://repository.unair.ac.id/94111/6/6.%20BAB%20III.pdf; http://repository.unair.ac.id/94111/7/7.%20BAB%20IV.pdf; http://repository.unair.ac.id/94111/8/8.%20BAB%20V.pdf; http://repository.unair.ac.id/94111/9/9.%20BAB%20VI.pdf; http://repository.unair.ac.id/94111/10/10.%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/94111/11/11.%20LAMPIRAN.pdf; http://repository.unair.ac.id/94111/; http://lib.unair.ac.id
• Institution: Universitas Airlangga

The anti microbial activities were determined by agar diffusion modified method by using Nutrient agar medium and Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Bacillus subtilis, Salmonella typhimurium as test microorganisms. Six spesies were showed anti microbial activity expressed as clear zone around the colonies agar (diameter 0,8 cm, height 3 mm). Diameters of the clear zone were observed each 24 hours for 10 days in order to establish diversity of the anti microbial activities. The profile of antimicrobial power of four Streptomyces sp. Isolates reveal different activities. Streptomyces sp. 2 has the highest activity in inhibiting Staphylococcus aureus ATCC 25922 at day 5 with inhibitory zone diameter as much as 29.2 mm, and Salmonella typhimurium at day 7 with inhibitory zone diameter as much as 27.5 mm, Streptomyces sp.-3 has the highest activity in inhibiting Pseudomonas aeruginosa TCC 27853 at day 6 with inhibitory zone diameter of 42.4 mm. Streptomycces sp.4 has the gighest activity in inhibiting Bacilluss subtilis at day 5 with inhibitory zone diameter of 20.7 mm. Phylogenetic analyses based on PCR showed that they formed distict phyletic band. Three isolates (sp.-1, sp.-3, and sp.-4) were determined based on their 16s rRna sequences. These isolates were assigned to genus Steptomyces. It is evident that by an partial 16s rRna gene sequence of the strain that it formed a distinc pyletic line within the range of variation encompassed by the genus Streptomycetes and the sharp separation of the organism from representatitves of the genus Streptomyces was strengthened by the fact that is sequencing 16S Rrna differed from those of 1589 recognized Streptomyces spesies. Key words: Streptomyces, mangrove, 16S rRNA gene, antibiotic