The Difference Between the Amount of Glucose as the Product of Metabolism of Glucosyltransferase Enzyme Streptococcus Mutans In Neutral pH and Optimal pH

• Main Authors: Nirawati Pribadi, Ruslan Effendy, Diva Amelia Ruslianda
• Format: Book Section PeerReviewed
• Language: English; English; English
• Published: Universiti Technology Malaysia. 2017
• Subjects: R Medicine; RK Dentistry
• Online Access: http://repository.unair.ac.id/94444/1/07_P33_The%20Difference%20Between%20the%20Amount%20of%20Glucose%20as%20the%20Product%20of%20Metabolism%20of%20Glucosyltransferase%20Enzyme%20Streptococcus%20Mutans%20In%20Neutral%20pH%20and%20Optimal%20pH.pdf; http://repository.unair.ac.id/94444/2/07_The%20Difference%20Between%20the%20Amount%20of%20Glucose%20as%20the%20Product%20of%20Metabolism%20of%20Glucosyltransferase%20Enzyme%20Streptococcus%20Mutans%20In%20Neutral%20pH%20and%20Optimal%20pH.pdf; http://repository.unair.ac.id/94444/3/07_The%20Difference%20Between%20the%20Amount%20of%20Glucose%20as%20the%20Product%20of%20Metabolism.pdf; http://repository.unair.ac.id/94444/
• Institution: Universitas Airlangga

Background:Caries happens due to the production of organic acid from the sugar metabolism, it cause a decrease in oral pH. The changes of pH affects the formation of glucan as the product of metabolism GTF enzyme S. mutans. Purpose: To investigate whether neutral pH (7) will decrease the amount of glucose compared to pH (5.5, 6.0, and 6,5). Methods: The S. mutans’s was cultured in BHI and centrifuged to obtain its supernatants. Six samples for each four group, consisted of 0.9 ml of 0.25 M sucrose in 0.2 M buffer, 0.1 ml of GTF enzyme, and buffer solution (pH 5.5, 6.0, 6.5, and 7) with total volume of 2ml (incubated), were then measured by using HPLC . Result: The amount of glucose (pH 7) are significantly less than samples (pH 5,5 and 6,5). Conclusion: The amount of glucose (pH 7) is less in acid pH, due to the changes of enzyme’s charge and denaturation.