Pengaruh Urea Dalam Media Maturasi In Vitro Terhadap Tingkat Maturasi Oosit Sapi

• Main Author: Sepvian Dewi Kurniawati
• Format: Theses and Dissertations NonPeerReviewed
• Language: English; English; English; English; English; English; English; English; English; English; English
• Published: 2020
• Subjects: TS2284-2288 Animal feeds and feed mills. Pet food industry
• Online Access: http://repository.unair.ac.id/94462/1/1.%20HALAMAN%20JUDUL.pdf; http://repository.unair.ac.id/94462/2/2.%20ABSTRACT.pdf; http://repository.unair.ac.id/94462/3/3.%20DAFTAR%20ISI.pdf; http://repository.unair.ac.id/94462/4/4.%20BAB%201%20PENDAHULUAN.pdf; http://repository.unair.ac.id/94462/5/5.%20BAB%202%20TINJAUAN%20PUSTAKA.pdf; http://repository.unair.ac.id/94462/6/6.%20BAB%203%20MATERI%20DAN%20METODE.pdf; http://repository.unair.ac.id/94462/7/7.%20BAB%204%20HASIL%20PENELITIAN.pdf; http://repository.unair.ac.id/94462/8/8.%20BAB%205%20PEMBAHASAN.pdf; http://repository.unair.ac.id/94462/9/9.%20BAB%206%20KESIMPULAN%20DAN%20SARAN.pdf; http://repository.unair.ac.id/94462/10/10.%20DAFTAR%20PUSTAKA.pdf; http://repository.unair.ac.id/94462/11/11.%20LAMPIRAN.pdf; http://repository.unair.ac.id/94462/; http://www.lib.unair.ac.id
• Institution: Universitas Airlangga

This study was aimed to determine the percentage of in vitro oocyte maturation in TCM – 199 media with urea. Cumulus oocyte complexes (COCs) that had been selected from the dissection media were added into the maturation media that contained TCM -199, Hepes, NaHCO3 and Kanamycine . Maturation media were added 0.15 IU/ml PMSG, 0.15 IU/ml hCG and FBS. Each petridish contained three drops of maturation media (300 μl/drops) which were coated with mineral oil and then incubated in a 5% CO2 incubator, with maximum humidity and temperature of 39˚C. Control (P0) was oocyte maturation without the addition of urea, while treatment 1 (P1) and treatment 2 (P2) were oocyte maturation with the addition of urea, 20 mg/dl and 40 mg/dl respectively. Aceto orcein staining showed that oocyte reached metaphase II phase were 51.25, 52.43 and 46.88 % respectively. ANOVA showed that there was no significant difference (p> 0.05) among treatment groups.