Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection
This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to “Quantiferon TB Gold In Tube” (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized “early secreted antigenic target 6kDa”...
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id-langga.949162020-03-10T01:51:51Z http://repository.unair.ac.id/94916/ Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection Rebekah Juniati Setiabudi Ni Made Mertaniasih Didik Handijatno Retno Asih Setyoningrum R Medicine (General) RZ Other systems of medicine This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to “Quantiferon TB Gold In Tube” (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized “early secreted antigenic target 6kDa” (ESAT-6) and “cultur filtrate protein 10kDa” (CFP-10) proteins to be induced on the specimen. ESAT-6 and CFP-10 are commercial products used to induce interferon gamma (INF-γ) which were to be read using sophisticated and expensive equipment. This study was intended to conduct an analysis on effective cocktail protein modification, i.e. ESAT-6, CFP-10 and Ag85A/B/C, with high validity to detect cellular immunity activity through in vitro examination on peripheral blood monocyte cells of Tuberculosis-suspected patients or patients with latent tuberculosis infection. Peripheral Blood Monocyte Cells (PBMCs) activity on children tuberculosis patient or Latent Tuberculosis Infection (LTBI), adult tuberculosis patient or LTBI, which induced by cocktail protein modification and not induced, were analyzed microscopically. The activity of PBMCs on children and adult tuberculosis patient or LTBI induced by RD1 secretory proteins: ESAT-6, CFP-10, Ag85A/B/C was higher compared to PBMCs which had not been induced by the secretory proteins. Cellular debris and monocyte cells with abnormal shapes were found on PBMCs which had been induced by RD1 secretory proteins at 8 th day after culture. Institute of Tropical Disease - Airlangga University 2016-01 Article PeerReviewed text en cc_by_nc_sa_4 http://repository.unair.ac.id/94916/1/Cellular%20Immunity%20Activation%20Method%20By%20Stimulating%20RD1%20Complex%20Proteins%20as%20Virulence%20Marker%20on%20Mycobacterium%20tuberculum%20to%20Establish%20Diagnosis%20on%20Tuberculosis%20and%20Latent%20Tuberculosis%20Infection.pdf text en http://repository.unair.ac.id/94916/2/Cellular%20Immunity%20Activation%20Method%20by%20.pdf text en http://repository.unair.ac.id/94916/3/Cellular%20Immunity%20Activation%20Method%20By%20Stimulating%20RD1%20Complex%20Proteins%20as%20Virulence%20Marker%20on%20Mycobacterium%20tuberculum%20to%20Establish%20Diagnosis%20on%20Tuberculosis%20and%20Latent%20Tuberculosis%20Infection.pdf Rebekah Juniati Setiabudi and Ni Made Mertaniasih and Didik Handijatno and Retno Asih Setyoningrum (2016) Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection. Indonesian Journal of Tropical and Infectious Disease, 6 (1). pp. 12-18. ISSN 2085-1103 https://e-journal.unair.ac.id/IJTID/article/view/1205 http://dx.doi.org/10.20473/ijtid.v6i1.1205 |
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R Medicine (General) RZ Other systems of medicine Rebekah Juniati Setiabudi Ni Made Mertaniasih Didik Handijatno Retno Asih Setyoningrum Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
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This study was intended to invent a simpler and more affordable method to establish diagnosis on Tuberculosis (TB) and Latent Tuberculosis infection (LTBI). Similar to “Quantiferon TB Gold In Tube” (QFT-GIT) and T.SPOT.TB methods, the researchers also utilized “early secreted antigenic target 6kDa” (ESAT-6) and “cultur filtrate protein 10kDa” (CFP-10) proteins to be induced on the specimen. ESAT-6 and CFP-10 are commercial products used to induce interferon gamma (INF-γ) which were to be read using sophisticated and expensive equipment. This study was intended to conduct an analysis on effective cocktail protein modification, i.e. ESAT-6, CFP-10 and Ag85A/B/C, with high validity to detect cellular immunity activity through in vitro examination on peripheral blood monocyte cells of Tuberculosis-suspected patients or patients with latent tuberculosis infection. Peripheral Blood Monocyte Cells (PBMCs) activity on children tuberculosis patient or Latent Tuberculosis Infection (LTBI), adult tuberculosis patient or LTBI, which induced by cocktail protein modification and not induced, were analyzed microscopically. The activity of PBMCs on children and adult tuberculosis patient or LTBI induced by RD1 secretory proteins: ESAT-6, CFP-10, Ag85A/B/C was higher compared to PBMCs which had not been induced by the secretory proteins. Cellular debris and monocyte cells with abnormal shapes were found on PBMCs which had been induced by RD1 secretory proteins at 8 th day after culture. |
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Article PeerReviewed |
author |
Rebekah Juniati Setiabudi Ni Made Mertaniasih Didik Handijatno Retno Asih Setyoningrum |
author_facet |
Rebekah Juniati Setiabudi Ni Made Mertaniasih Didik Handijatno Retno Asih Setyoningrum |
author_sort |
Rebekah Juniati Setiabudi |
title |
Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
title_short |
Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
title_full |
Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
title_fullStr |
Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
title_full_unstemmed |
Cellular Immunity Activation Method By Stimulating RD1 Complex Proteins as Virulence Marker on Mycobacterium tuberculum to Establish Diagnosis on Tuberculoss and Latent Tuberculosis Infection |
title_sort |
cellular immunity activation method by stimulating rd1 complex proteins as virulence marker on mycobacterium tuberculum to establish diagnosis on tuberculoss and latent tuberculosis infection |
publisher |
Institute of Tropical Disease - Airlangga University |
publishDate |
2016 |
url |
http://repository.unair.ac.id/94916/1/Cellular%20Immunity%20Activation%20Method%20By%20Stimulating%20RD1%20Complex%20Proteins%20as%20Virulence%20Marker%20on%20Mycobacterium%20tuberculum%20to%20Establish%20Diagnosis%20on%20Tuberculosis%20and%20Latent%20Tuberculosis%20Infection.pdf http://repository.unair.ac.id/94916/2/Cellular%20Immunity%20Activation%20Method%20by%20.pdf http://repository.unair.ac.id/94916/3/Cellular%20Immunity%20Activation%20Method%20By%20Stimulating%20RD1%20Complex%20Proteins%20as%20Virulence%20Marker%20on%20Mycobacterium%20tuberculum%20to%20Establish%20Diagnosis%20on%20Tuberculosis%20and%20Latent%20Tuberculosis%20Infection.pdf http://repository.unair.ac.id/94916/ https://e-journal.unair.ac.id/IJTID/article/view/1205 http://dx.doi.org/10.20473/ijtid.v6i1.1205 |
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