The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast
Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp‑capping but still has a high failure rate. Thus, an alternative material is needed, one of which is propolis. The combination of Ca(OH)2 propolis is expected to have better quality and to be biocompatible. The aim of this study...
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2020
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id-langga.949442020-03-19T04:44:23Z http://repository.unair.ac.id/94944/ The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast Ira Widjiastuti Mieke Kusuma Dewi Edhi Arief Prasetyo Nirawati Pribadi Mochamad Moedjiono R Medicine RK Dentistry Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp‑capping but still has a high failure rate. Thus, an alternative material is needed, one of which is propolis. The combination of Ca(OH)2 propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16‑year‑old patients, were divided into seven groups: Group 1 (10 µg Ca(OH)2); Group 2 (10 µg propolis); Group 3 (15 µg propolis); Group 4 (20 µg propolis); Group 5 (Ca(OH)2‑propolis 1:1); Group 6 (Ca(OH)2‑propolis 1:1.5); and Group 7 (calcium hydroxide‑propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene’s test, one‑way analysis of variance, and Tukey‑honestly significant difference (P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH)2 and propolis combination is greater than the application of Ca(OH)2 or propolis with significant different between groups (P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH)2‑propolis combination is greater than that of the application of Ca(OH)2 and propolis alone. Wolters Kluwer - Medknow 2020 Article PeerReviewed text en http://repository.unair.ac.id/94944/1/05_JAdvPharmTechRes11120-5445201_013045.pdf text en http://repository.unair.ac.id/94944/2/05_The%20cytotoxicity%20test%20of%20cal.pdf text id http://repository.unair.ac.id/94944/3/Bukti%2005.pdf Ira Widjiastuti and Mieke Kusuma Dewi and Edhi Arief Prasetyo and Nirawati Pribadi and Mochamad Moedjiono (2020) The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast. Journal of Advanced Pharmaceutical Technology & Research, 11 (1). pp. 20-24. ISSN 0976-2094 |
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R Medicine RK Dentistry Ira Widjiastuti Mieke Kusuma Dewi Edhi Arief Prasetyo Nirawati Pribadi Mochamad Moedjiono The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
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Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp‑capping but still has a high failure rate. Thus, an alternative material is needed, one of which is propolis. The combination of Ca(OH)2 propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16‑year‑old patients, were divided into seven groups: Group 1 (10 µg Ca(OH)2); Group 2 (10 µg propolis); Group 3 (15 µg propolis); Group 4 (20 µg propolis); Group 5 (Ca(OH)2‑propolis 1:1); Group 6 (Ca(OH)2‑propolis 1:1.5); and Group 7 (calcium hydroxide‑propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene’s test, one‑way analysis of variance, and Tukey‑honestly significant difference (P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH)2 and propolis combination is greater than the application of Ca(OH)2 or propolis with significant different between groups (P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH)2‑propolis combination is greater than that of the application of Ca(OH)2 and propolis alone. |
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Article PeerReviewed |
author |
Ira Widjiastuti Mieke Kusuma Dewi Edhi Arief Prasetyo Nirawati Pribadi Mochamad Moedjiono |
author_facet |
Ira Widjiastuti Mieke Kusuma Dewi Edhi Arief Prasetyo Nirawati Pribadi Mochamad Moedjiono |
author_sort |
Ira Widjiastuti |
title |
The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
title_short |
The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
title_full |
The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
title_fullStr |
The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
title_full_unstemmed |
The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
title_sort |
cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide‑propolis combination in human pulp fibroblast |
publisher |
Wolters Kluwer - Medknow |
publishDate |
2020 |
url |
http://repository.unair.ac.id/94944/1/05_JAdvPharmTechRes11120-5445201_013045.pdf http://repository.unair.ac.id/94944/2/05_The%20cytotoxicity%20test%20of%20cal.pdf http://repository.unair.ac.id/94944/3/Bukti%2005.pdf http://repository.unair.ac.id/94944/ |
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