Cytotoxicity Assay of Sodium Hypochlorite and QMix on Cultured Human Periodontal Ligament Fibroblast Cells

• Main Authors: Dian Agustin Wahjuningrum, Makkunrai Eka Kramatawati Elizabeth, Fikarini Hadi Puteri, Andi Ainul Mardiyah, Ari Subiyanto
• Format: Article PeerReviewed
• Language: English; English; Indonesian
• Published: Medknow Publications and Media Pvt, Ltd. 2019
• Subjects: R Medicine; RK Dentistry
• Online Access: http://repository.unair.ac.id/95231/1/02.%20021142.pdf; http://repository.unair.ac.id/95231/2/2.Cytotoxicity%20assay%20of%20sodium%20hypochlorite%20and%20QMix%20on%20cultured%20human%20periodontal%20ligament%20fibroblast%20cells%282%29.pdf; http://repository.unair.ac.id/95231/3/2.validasi%20%28Cytotoxicity%29.pdf; http://repository.unair.ac.id/95231/; http://www.jioh.org/text.asp?2019/11/4/204/264433; https://doi.org/10.4103/jioh.jioh_48_19
• Institution: Universitas Airlangga

Aims and Objectives: Sodium hypochlorite (NaOCl) and QMix are solutions used for root canal irrigation. During the endodontic procedure, irrigation solutions are often leaked beyond the apical foramen and as a result cause periapical tissue complication because of their toxicity. This study was aimed at proving the cytotoxicity effect of NaOCl and QMiX on cultured human periodontal ligament fibroblast cells (HPDLFc). Materials and Methods: HPDLFc were exposed to NaOCl and QMix at various concentrations. Cell viability was assessed with Mosmann’s tetrazolium toxicity assay and the results were measured and statistically analyzed by probit analysis to determine the median lethal concentration (LC50), the concentration that is lethal to 50% of the cells and therefore considered cytotoxic. Results: NaOCl and QMix are cytotoxic to HPDLFc at 0.254 and 0.363 μL/mL, respectively. Conclusion: Both solutions are cytotoxic to HPDLFc at certain concentrations