Melatonin as an Antioxidant in Experimental Diabetes Mellitus Type 1

• Main Authors: Boedi Setiawan, Hartanta Barus, Teresia Ayu Yuliamarini Banjarnahor
• Format: Book Section PeerReviewed
• Language: English; English; English
• Published: Universiti Putra Malaysia Press 2009
• Subjects: SF405.5-407 Laboratory animals; SF811-909 Veterinary medicine of special organs, regions, and systems
• Online Access: http://repository.unair.ac.id/95242/2/Bukti%20C%2013%20Melatonin%20as%20an%20Antioxidant%20in%20Experimental%20....pdf; http://repository.unair.ac.id/95242/1/Peer%20review%20C%2013.%20Melatonin%20as%20an%20Antioxidant%20in%20....pdf; http://repository.unair.ac.id/95242/3/Turnitin%20C%2013%20Melatonin%20as%20an%20Antioxidant%20in%20Experimental%20....pdf; http://repository.unair.ac.id/95242/
• Institution: Universitas Airlangga

The aim of this research was to study the effect of melatonin as antioxidant like in experimental diabetes mellitus type I .Thirty rats were divided into five groups P0, P1 , P2, P3, and P4. Group P0 as a negative control of diabetes were injected with normal saline 0.5 cc and group P1 (a positive control) were injected with alloxan 150 mg/kg without melatonin. Group P2, P3, and P4 received melatonin at 5 mg/kg, 10 mg/kg and 15 mg/ kg, respectively, for seven days, then on the eighth day alloxan was given at 150 mg/kg to induce diabetes. Blood samples were collected four days after alloxan injection to measure the level of blood glucose and histopatological change of langerhans cells. The data was analyzed by using ANOVA and continued with Duncan's Multiple Range Test. The result of the experiment showed that melatonin (P2, P3 and P4) did not cause hyperglycemia, thus melatonin could decrease the damage to the Langerhans cells. It could be concluded that Melatonin can be used to prevent damage to pancreas from free radical in male wistar rat (Rattus novergicus). Keywords: melatonin, alloxan, hyperglycemia, Langerhans cells