Influence of Extract Propolis on the Adherence of Enterococcus faecalis as a Candidate Root Canal Irrigation Solution

• Main Authors: Dian Agustin Wahjuningrum, Ari Subijanto
• Format: Article PeerReviewed
• Language: English; English; Indonesian
• Published: IAMURE Multidisciplinary Research 2015
• Subjects: R Medicine; RK Dentistry
• Online Access: http://repository.unair.ac.id/95289/1/9.validasi%20%28Influenza%20of%20Extact%29.pdf; http://repository.unair.ac.id/95289/2/9.Influence%20of%20Extract%20Propolis%20on%20the%20Adherence%20of%20Enterococcus%20faecalis%20as%20a%20Candidate%20Root%20Canal%20Irrigation%20Solution.pdf; http://repository.unair.ac.id/95289/3/09.%20Influence%20of%20Extract%20Propolis.pdf; http://repository.unair.ac.id/95289/
• Institution: Universitas Airlangga

Failure of endodontic treatment is commonly related to the presence of persistent microorganisms. Enterococcus faecalis can form a calcified biofilm in tough nvironmental conditions within the root canals. Biofilm-mediated infections can be caused by biofilm formation on tissues and biomaterial surfaces which are difficult to treat as a result of the increased antimicrobial esistance of biofilm bacteria. The bacteria that are close to a solid surface forms the initial and the most important step in the formation of biofilm. Propolis, natural bio product from bee contains tt-farnesol and apigenin that have mechanisms for inhibiting adherence of bacterial biofilm. This study assessed the influence of extract Propolis on from the adherence of Enterococcus faecalis. This study was designed as post-test only control group laboratory experiment. The Propolis 8-16% was extracted by using maceration method and serial dilution. Susceptibilities to the test extracts were analyzed using the agar diffusion method and by etermining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC); the effect on bacterial adherence to a glass surface was also assessed. With the use of the one-way ANOVA with multiple comparisons, significantly fewer bacteria were found adhering to the extract Propolis. AlI treatment produced the maximum reduction in adherence of E. faecalis to smooth surface followed by 8-16%. Extract Propolis 14% show effectiveness in preventing adhesion of E. faecalis.