Effect of Vitamin E (α-Tocopherol) Administration on Sperm Motility and Morphology of Sprague Dawley Strain Rats After Cisplatin Treatment

• Main Authors: Dimas Visa Aditya, Tarmono Djojodimedjo, Doddy M. Soebadi
• Format: Article PeerReviewed
• Language: English; English; English
• Published: The Indonesian Urological Association 2019
• Subjects: R Medicine (General); RD Surgery
• Online Access: http://repository.unair.ac.id/95454/1/Effect%20of%20Vitamin%20E.pdf; http://repository.unair.ac.id/95454/2/Effect%20of%20Vitamin%20E%20%28%CE%B1-Tocopherol%29%20Administration%20on%20Sperm%20Motility%20and%20Morphology%20of%20Sprague%20Dawley%20Strain%20Rats%20After%20Cisplatin%20Treatment.pdf; http://repository.unair.ac.id/95454/3/Effect%20of%20Vitamin%20E%20%28%CE%B1-Tocopherol%29%20Administration%20on%20Sperm%20Motility%20and%20Morphology%20of%20Sprague%20Dawley%20Strain%20Rats%20After%20Cisplatin%20Treatment_compressed.pdf; http://repository.unair.ac.id/95454/; http://juri.urologi.or.id/juri/article/view/539; https://doi.org/10.32421/juri.v26i1.539
• Institution: Universitas Airlangga

Objective: To evaluate the protective effects of vitamin E α-tocopherol isomer against the toxicity of cisplatin on sperm motility and morphology in Sprague Dawley rats. Material & Methods: Twenty-four rats were grouped into four groups (n=6). The control group (CN) was injected with normal saline, second group (CP) was injected with cisplatin, the third group (P1) was injected with cisplatin and vitamin E 50 mg/kgBW for 7 weeks P.O, the fourth group (P2) was injected with cisplatin and vitamin E 200 mg/kgBW for 7 weeks P.O. Vitamin E was given from 3 weeks before cisplatin injection and 4 weeks following cisplatin injection. At 7th week, all the samples were undergoing bilateral orchidectomy. Vitamin E that being used in this study was α-tocopherol isomer. Results: Cisplatin decreased motility and morphology of spermatozoa significantly against controls. Vitamin E 50 mg/kgBW and 200 mg/kgBW significantly increased motility of spermatozoa (p<0.05) compared to those in the cisplatin group only. Vitamin E 50 mg/kgBW, and 200 mg/kgBW did not have a significant difference in spermatozoa motility compare to control groups. Vitamin E 50 mg/kgBW and 200 mg/kgBW could increase the spermatozoa morphology significantly compare to those cisplatin only group. Vitamin E 50 mg/kgBW, and 200 mg/kgBW did not have a significant difference in spermatozoa morphology compared to control groups. Conclusion: α-tocopherol 50 mg/kgBW and 200 mg/kgBW provided a same protective effect against spermatozoa damage especially in motility and morphology aspect due to cisplatin exposure. Therefore, in this study it was more recommended to use α-tocopherol in 50 mg/kgBW dose than 200 mg/kgBW.