Neuroprotective Effect Of Citicholine In Mercury Intoxication

• Main Authors: Paulus Sugianto, Aulanni'am, M. Aris Widodo, Mohammad Hasan Machfoed
• Format: Article PeerReviewed
• Language: English; English; English
• Published: University Grants Commission UGC Approved Journal 2013
• Subjects: R Medicine (General); RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
• Online Access: http://repository.unair.ac.id/97837/1/Neuroprotective%20Effect%20of%20Citicholine%20in%20Mercury%20Intoxication.pdf; http://repository.unair.ac.id/97837/2/Neuroprotective%20Effect%20.pdf; http://repository.unair.ac.id/97837/3/Neuroprotective%20Effect%20of%20Citicholine%20in%20Mercury%20Intoxication.pdf; http://repository.unair.ac.id/97837/; http://www.ijpsi.org/Papers/Vol2(11)/G0211038044.pdf
• Institution: Universitas Airlangga

Objective: Mercury induces cell death in various cell lines by apoptosis or necrosis. It is associated with alteration of some apoptosis regulators such as p53 and caspase-3. Citicholin has been widely used as neuroprotectant in cerebrovascular disease and other neurologic disorder. Previous studies have shown beneficial effect of citicholin in reducing inflammation and apoptosis in patient with stroke and pain, but none of those have used mercury intoxicated rat animal model. We try to investigate the neuroprotective effect of citicholin mercury intoxication. Methods : We conducted an controlled study using male rats (Rattus Novergicus) as animal model and devided the subjects into 5 groups (P0,P1,P2,P3,P4) and 0.4 mg/kg BW of MeHgCl3 is administered for 21 days to all groups. Citicholin (0,5,10,20 and 40 mg/kgBW/day was added during the exposure. Protein p53 and Caspase-3 expression on neurons were counted. Result : The mean scores±SD of p53 in group P0;P1;P2;P3;P4 were 8,406±0,70; 6,828±1,40; 4,680±1,2; 1,752±0,4; 0,807±0,1 respectively and for Caspase 3 were 8,854±1,91; 6.670±0,61; 4,21±1,02; 2,632±1,00; 0,818± 0,22 respectively. Conclusion : citicoline treatment exerts neuroprotective effect against neuronal apoptotic changes by reducing the expression of p53 and caspase-3 in dose dependent manner on mercury intoxicated rat.