A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical

• Main Authors: a Tin Myo Thant, Nanik Siti Aminah, Alfinda Novi Kristanti, Rico Ramadhan, Preecha Phuwapraisirisan, Yoshiaki Takaya
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: Penerbit Taylor & Francis 2019
• Subjects: Q Science (General); QD Chemistry
• Online Access: http://repository.unair.ac.id/98011/1/C05.%20Abstract.pdf; http://repository.unair.ac.id/98011/2/C05.%20Fulltext%20-%20A%20new%20pyrano%20coumarin%20from%20Clausena%20excavata%20roots%20displaying.pdf; http://repository.unair.ac.id/98011/3/C05.%20Peerreviewer%20-%20A%20new%20pyrano%20coumarin%20from%20Clausena.pdf; http://repository.unair.ac.id/98011/4/C05.%20Similarity%20-%20A%20new%20pyrano%20coumarin%20from%20Clausena%20excavata%20roots%20displaying%20-%20Copy.pdf; http://repository.unair.ac.id/98011/; https://www.tandfonline.com/doi/abs/10.1080/14786419.2019.1586696?journalCode=gnpl20; https://doi.org/10.1080/14786419.2019.1586696
• Institution: Universitas Airlangga

A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC50 values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC50 5.45 µM) and sucrase (IC50 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC50 values 1.92 and 5.58 mM.