Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy
Aims: This research aimed to determine the efficacy of Syzygium cumini L. as an adjuvant therapy on blood changes and splenic index of mice model malaria. Materials and Methods: Mice were infected intraperitoneally with 0.2 ml red blood cell (RBC) that contains 1×106 Plasmodium berghei. 35 mice w...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article PeerReviewed |
Language: | English Indonesian English English |
Published: |
Veterinary World
2019
|
Subjects: | |
Online Access: | https://repository.unair.ac.id/98381/2/Bukti%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf https://repository.unair.ac.id/98381/3/Peer%20review%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf https://repository.unair.ac.id/98381/1/Bukti%20C%2005%20Hematologic%20changes%20and%20splenic%20index%20on%20....pdf https://repository.unair.ac.id/98381/6/%28Bukti%20Korespondensi%29%20Hematologic%20Changes%20and%20Splenic%20Index%20on%20Malaria%20Mice%20Models%20Given%20Syzygium%20cumini%20as%20an%20Adjuvant%20Therapy.pdf https://repository.unair.ac.id/98381/ http://www.veterinaryworld.org/Vol.12/January-2019/15.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universitas Airlangga |
Language: | English Indonesian English English |
id |
id-langga.98381 |
---|---|
record_format |
dspace |
spelling |
id-langga.983812021-10-07T06:45:35Z https://repository.unair.ac.id/98381/ Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy Lilik Maslachah, - Rahmi Sugihartuti, - Retno Sri Wahyuni, - SF811-909 Veterinary medicine of special organs, regions, and systems Aims: This research aimed to determine the efficacy of Syzygium cumini L. as an adjuvant therapy on blood changes and splenic index of mice model malaria. Materials and Methods: Mice were infected intraperitoneally with 0.2 ml red blood cell (RBC) that contains 1×106 Plasmodium berghei. 35 mice were divided into seven treatment groups: Group K0: Mice were not infected; K1: Mice were infected; K2: Mice were infected and given chloroquine; P1: Mice were infected and given S. cumini leaf extract; P2: Mice were infected and given chloroquine and also S. cumini leaf extract; P3: Mice was infected and given S. cumini stem bark extract; and P4: Mice were infected and given chloroquine and S. cumini stem bark extract. Treatment was given for 4 days 24 h post-P. berghei infection. 21st day post-P. berghei infection, blood was taken from the heart for hematological examination, and the spleen was taken to examine the splenic index and also to measure the weight and length of the spleen. Hematological data and splenic index were analyzed by analysis of variance test, and if there is a difference, the test is continued by Duncan’s multiple range test with 5% level. Results: The K0 group has normal hemoglobin (HGB), RBC, and hematocrit (HCT) and significantly different (p<0.05) than other groups. HGB, RBC, and HCT of K1 group were under normal range, lowest, and significantly different (p<0.05) than other groups. Mean corpuscular volume and mean corpuscular HGB values of K2 groups showed a decrease. The number of leukocytes, lymphocytes, and monocytes of K1 groups was increasing and significantly different (p<0.05) with K2 and treatment group. The length, width, weight, and splenic index of K1 group were significantly different (p<0.05) with K0 group. K2 and treatment groups showed that the length and width of spleens were significantly different (p<0.05) with K1. Conclusion: The combination of chloroquine with leaf and chloroquine with stem bark extract of S. cumini as adjuvant therapy may increase the amount of erythrocyte; decrease the number of leukocytes, lymphocytes, and monocytes; and decrease the length, width, and splenic index on malaria mice models. Keywords: hematology, splenic index, Syzygium cumini, Plasmodium berghei Veterinary World 2019-01 Article PeerReviewed text en https://repository.unair.ac.id/98381/2/Bukti%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf text id https://repository.unair.ac.id/98381/3/Peer%20review%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf text en https://repository.unair.ac.id/98381/1/Bukti%20C%2005%20Hematologic%20changes%20and%20splenic%20index%20on%20....pdf text en https://repository.unair.ac.id/98381/6/%28Bukti%20Korespondensi%29%20Hematologic%20Changes%20and%20Splenic%20Index%20on%20Malaria%20Mice%20Models%20Given%20Syzygium%20cumini%20as%20an%20Adjuvant%20Therapy.pdf Lilik Maslachah, - and Rahmi Sugihartuti, - and Retno Sri Wahyuni, - (2019) Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy. Veterinay World, 12 (01). pp. 106-111. ISSN 0972-8988, e-ISSN 2231-0916 http://www.veterinaryworld.org/Vol.12/January-2019/15.pdf 10.14202/vetworld.2019.106-111 |
institution |
Universitas Airlangga |
building |
Universitas Airlangga Library |
continent |
Asia |
country |
Indonesia Indonesia |
content_provider |
Universitas Airlangga Library |
collection |
UNAIR Repository |
language |
English Indonesian English English |
topic |
SF811-909 Veterinary medicine of special organs, regions, and systems |
spellingShingle |
SF811-909 Veterinary medicine of special organs, regions, and systems Lilik Maslachah, - Rahmi Sugihartuti, - Retno Sri Wahyuni, - Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
description |
Aims: This research aimed to determine the efficacy of Syzygium cumini L. as an adjuvant therapy on blood changes and splenic index of mice model malaria.
Materials and Methods: Mice were infected intraperitoneally with 0.2 ml red blood cell (RBC) that contains 1×106 Plasmodium berghei. 35 mice were divided into seven treatment groups: Group K0: Mice were not infected; K1: Mice were infected; K2: Mice were infected and given chloroquine; P1: Mice were infected and given S. cumini leaf extract; P2: Mice were infected and given chloroquine and also S. cumini leaf extract; P3: Mice was infected and given S. cumini
stem bark extract; and P4: Mice were infected and given chloroquine and S. cumini stem bark extract. Treatment was given for 4 days 24 h post-P. berghei infection. 21st day post-P. berghei infection, blood was taken from the heart for hematological examination, and the spleen was taken to examine the splenic index and also to measure the weight and length of the spleen. Hematological data and splenic index were analyzed by analysis of variance test, and if there is a difference, the test is continued by Duncan’s multiple range test with 5% level.
Results: The K0 group has normal hemoglobin (HGB), RBC, and hematocrit (HCT) and significantly different (p<0.05) than other groups. HGB, RBC, and HCT of K1 group were under normal range, lowest, and significantly different (p<0.05) than other groups. Mean corpuscular volume and mean corpuscular HGB values of K2 groups showed a decrease. The number of leukocytes, lymphocytes, and monocytes of K1 groups was increasing and significantly different (p<0.05) with K2 and treatment group. The length, width, weight, and splenic index of K1 group were significantly different (p<0.05) with K0 group. K2 and treatment groups showed that the length and width of spleens were significantly different (p<0.05) with K1.
Conclusion: The combination of chloroquine with leaf and chloroquine with stem bark extract of S. cumini as adjuvant therapy may increase the amount of erythrocyte; decrease the number of leukocytes, lymphocytes, and monocytes; and decrease the length, width, and splenic index on malaria mice models.
Keywords: hematology, splenic index, Syzygium cumini, Plasmodium berghei |
format |
Article PeerReviewed |
author |
Lilik Maslachah, - Rahmi Sugihartuti, - Retno Sri Wahyuni, - |
author_facet |
Lilik Maslachah, - Rahmi Sugihartuti, - Retno Sri Wahyuni, - |
author_sort |
Lilik Maslachah, - |
title |
Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
title_short |
Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
title_full |
Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
title_fullStr |
Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
title_full_unstemmed |
Hematologic Changes and Splenic Index on Malaria Mice Models Given Syzygium cumini as an Adjuvant Therapy |
title_sort |
hematologic changes and splenic index on malaria mice models given syzygium cumini as an adjuvant therapy |
publisher |
Veterinary World |
publishDate |
2019 |
url |
https://repository.unair.ac.id/98381/2/Bukti%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf https://repository.unair.ac.id/98381/3/Peer%20review%20C%2005%20Hematologic%20Changes%20and%20Splenic%20....pdf https://repository.unair.ac.id/98381/1/Bukti%20C%2005%20Hematologic%20changes%20and%20splenic%20index%20on%20....pdf https://repository.unair.ac.id/98381/6/%28Bukti%20Korespondensi%29%20Hematologic%20Changes%20and%20Splenic%20Index%20on%20Malaria%20Mice%20Models%20Given%20Syzygium%20cumini%20as%20an%20Adjuvant%20Therapy.pdf https://repository.unair.ac.id/98381/ http://www.veterinaryworld.org/Vol.12/January-2019/15.pdf |
_version_ |
1713213796926881792 |