Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model

• Main Authors: Laili Muninggar, Widjiati, Indra Yuliati, Brahmana Askandar, Poedjo Hartono
• Format: Article PeerReviewed
• Language: English; English; English
• Published: National Cancer Center Dharmais Cancer Hospital 2018
• Subjects: R Medicine (General); RG Gynecology and obstetrics
• Online Access: http://repository.unair.ac.id/98797/1/Effects%20of%20Curcumin%20on%20Vascular%20Endothelial%20Growth%20Factor%20Expression%20on%20Rattus%20norvegicusCervical%20Cancer%20Xenograft%20Model.pdf; http://repository.unair.ac.id/98797/2/Effects%20of%20Curcumin.pdf; http://repository.unair.ac.id/98797/3/Effects%20of%20Curcumin%20on%20Vascular%20Endothelial%20Growth%20Factor%20Expression%20on%20Rattus%20norvegicusCervical%20Cancer%20Xenograft%20Model.pdf; http://repository.unair.ac.id/98797/; https://www.indonesianjournalofcancer.or.id/e-journal/index.php/ijoc/article/view/616; http://dx.doi.org/10.33371/ijoc.v12i3.616
• Institution: Universitas Airlangga

Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model. Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression. Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05). Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.