The Ability Of Immunoglobulin Y From Porphyromonas Gingivalis To Prevent Adhesion Of Fusobacterium Nucleatum And Aggregatibacter
Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Porphyromonas gingivalis are the periodontitis bacterium. IgY is a type of immunoglobulin that found in poultry, such as: chicken and birds. IgY can be used as an alternative prevention of plaque accumulation, which can cause chronic...
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| Main Authors: | , , , |
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| Format: | Article PeerReviewed |
| Language: | English English Indonesian English |
| Published: |
DR. P. R. YADAV
2020
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| Subjects: | |
| Online Access: | http://repository.unair.ac.id/99728/1/07_THE%20ABILITY%20OF%20IMMUNOGLOBULIN%20Y%20FROM.pdf http://repository.unair.ac.id/99728/2/07_Turnitin%2011%20The%20ability%20of%20immunoglobulin%20Y%20from%20porphyromonas.pdf http://repository.unair.ac.id/99728/6/validasi%207.pdf http://repository.unair.ac.id/99728/8/The%20Ability%20Of%20Immunoglobulin%20Y%20From.pdf http://repository.unair.ac.id/99728/ |
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| Institution: | Universitas Airlangga |
| Language: | English English Indonesian English |
| Summary: | Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Porphyromonas gingivalis are the periodontitis bacterium. IgY is a type of immunoglobulin that found in poultry, such as: chicken and birds. IgY can be used as an alternative prevention of plaque accumulation, which can cause chronic periodontitis. IgY is attractive for oral immunotherapy due to its several properties. The aim of this study was to prove IgY in egg yolk ability that can prevent the adhesion of Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans bacteria on enterocyte cell. The sample was divided into 8 groups, each group containing 10 ml of Porphyromonas gingivalis IgY and 50 enterocyte cells. The control group contained 50 ml of Porphyromonas gingivalis IgY and 50 ml of enterocyte cells. The first group to the seventh group was performed serial dilution with the first group containing 90 ml PBS and 10 ml Porphyromonas gingivalis IgY, the second group to the seventh group containing 50 PBS before adding 50 ml of enterocyte cells and 50 ml of bacterial suspension per group. The inherent bacterial count was calculated using a light microscope and the adherence index value was calculated. This study shows that Porphyromonas gingivalis IgY can significantly reduce the adherence index value of Aggregatibacter actinomycetemcomitans and can reduce the adherence index value of Fusobacterium nucleatum but not significantly.
Porphyromonas gingivalis IgY can inhibit. Aggregatibacter actinomycetemcomitans adherence, but cannot inhibit Fusobacterium nucleatum adherence. |
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