IDENTIFIKASI MOLEKULAR ISOLAT BAKTERI DARI SPONS LAUT PENGHASIL SENYAWA ANTIPLASMODIUM
Bacteria from sponges are one source of bioactive compounds. One of them is known as an antiplasmodial. This research was conducted to evaluate the antiplasmodial activity of compounds produced by bacteria from sponges, classified among bacteria from sponges were active as antiplasmodial by Rep- PCR...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/118667/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58641 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Bacteria from sponges are one source of bioactive compounds. One of them
is known as an antiplasmodial. This research was conducted to evaluate the
antiplasmodial activity of compounds produced by bacteria from sponges,
classified among bacteria from sponges were active as antiplasmodial by Rep-
PCR, identified the species of bacteria based on the analysis 16S rRNA gene and
characterize the compounds of the active extract antiplasmodial. Bacteria
fermented and extracted with ethyl acetate which then assesed for heme
polymerization inhibition assay and in vitro antiplasmodial activity assay.
BOXA1R primer was used to analyze the Rep-PCR, while 27F and 1385R
primers were used to analyze the 16S rRNA gen among bacteria were active
antiplasmodial. Liquid chromatography-mass spectrometry (LC-MS) was used to
analyze the compounds of the active extract antiplasmodial Among ten bacteria
isolated examined, seven isolates showed good activity in heme polimerization
assay. Among them, four isolates i.e BYT7, 102TP, BYT5C4 and BYT5C5 has
moderate activity in vitro assay with IC50 value respectively 32,87±2,55 |
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