SINTESIS Cu(II)/SILIKA DENGAN METODE SOL-GEL SEBAGAI MATERIAL ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus

• Main Authors: , LISA TANIA, , Prof. Dr. Karna Wijaya, M.Eng.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/119306/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59302
• Institution: Universitas Gadjah Mada

Synthesis of Cu(II)/silica by sol-gel method as antibacterial material against Escherichia coli (E.coli) and Staphylococcus auerus (S.aureus) has been done. The sol-gel process was carried out by reacting TEOS, H2O, HCl, etilen glikol and CuCl2.2H2O. Heat treatment were done by varying the combination of microwave and oven. Aging processes were done for 24h. The Cu(II)/silica was then characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction method (XRD), and Transmission Electron Microscopy (TEM). The Cu(II) ions concentration that released from Cu(II)/silica was determined by AAS. Cu content in Cu(II)/silica was determined by X-Ray Fluoresence (XRF). Porosity of materials were determine by monolayer adsorption of N2 gas. Antibacterial test was done by calculate the number of bacteria surviving after contacted with Cu(II)/silica by plate count method. The XRF analysis results showed that CuCl2.2H2O 0.25 M produced the optimum Cu content 38.4% in Cu(II)/silica. Cu(II)/silica synthesized by heating in the oven at 150oC for 3 h after dried by microwave and aged resulted the optimum Cu(II)/silica due to the least release of Cu(II) ions from Cu(II)/silica when analyzed by AAS (10.35%). The Cu(II)/silica with concentration of 163 ppm could kill E.coli and S.aureus from concentration of 1.5 x106CFU/mL to 0 CFU/mL in 2 h contact time. On the other hand, silica with the same concentration to that of the Cu(II)/silica showed no antibacterial effect against E.coli and S.aureus.