PERBEDAAN EKSPRESI INTERLEUKIN-6 OLEH MAKROFAG PADA JARINGAN KANKER KOLOREKTAL DAN JARINGAN NORMAL PADA PASIEN DENGAN KANKER KOLOREKTAL

• Main Authors: , Pebri Rahadian Ramlie, , Prof. dr. Siti Nurdjanah, M.Kes, SpPD-KGEH
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/119342/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59338
• Institution: Universitas Gadjah Mada

INTERLEUKIN-6 EXPRESSION DIFFERENCES BY MACHROPHAGE IN COLORECTAL CANCER TISSUE AND NORMAL TISSUE IN PATIENTS WITH COLORECTAL CANCER Pebri Rahadian R, Siti Nurdjanah, Neneng Ratnasari Department of Internal Medicine-Scholl of Medicine Gadjah Mada University/Sardjito General Hospital Yogyakarta Colorectal cancer is the second cause of cancer deaths in the United States (U.S.), approximately 50,000 men and women every year. This cancer occurs in 5% of the adult population in the U.S. and half causing death, in Indonesia there has been no data on the epidemiology, according to medical records in Dr. Sardjito Yogyakarta there are 119 (out of 28412 patients) of patients in 2010 and 124 (from around 28 796 patients) in 2011 with respectively 3 and 9 patients died during treatment (unpublished). However, the molecular pathogenesis of these cancers is still poorly understood. Interleukin - 6 ( IL - 6 ) is a pleiotropic cytokine that has a physiological function , stimulates the activity of B cells produce immunoglobulin and serves as the hepatocyte stimulating factor , as a B cell growth are synthesized by various cell types , including T cells , macrophages and stromal cells in response to stimulate tumor necrosing factor α ( TNF - α ) and interleukin - 1 ( IL - 1 ) . Activation of the IL - 6 complex activates Janus Kinase ( JAK ) , signal transducer and activators of transcription ( STAT ) that regulate proliferation and apoptosis . The purpose of this study was to determine whether there are differences in the expression of IL - 6 by macgropage in colorectal cancer tissue compared with healthy tissue in the same patient . Histological tissue samples were paraffin blocks in unit Patolodi Anatomy of patients with colorectal cancer who had undergone surgery at Dr . Sardjito Yogyakarta between January 2010 and December 2011 who meet the inclusion and exclusion criteria as much as 25 patient tissue , samples were selected to sample size is met . Of samples stained with Histo Chemistry Imunno staining ( IHC ) was obtained tissue samples of 14 female and 11 tissue samples of male sex , age of the youngest 39 years old and the oldest was 84 years old . Mean value for IL - 6 expression in normal tissues is 0.2616 and 0.3924 for the cancerous tissue is . Obtained significant differences in the expression of IL - 6 both tissue macrophages with p = 0.001 . In this study also found a cut-off point IL - 6 expression in macrophages at 0.35 with a value of sensitivity =72 %, specivitivity = 72 %, positive predictiv value = 72% and negatif predictiv value = 72 %.