ANALISIS SEKUEN PARSIAL GEN MAJOR CAPSID PROTEIN (MCP) INFECTIOUS MYONECROSIS VIRUS (IMNV) ISOLAT INDONESIA

• Main Authors: , ROZI, , Prof. Dr. drh. Kurniasih, M.V.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/119795/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59799
• Institution: Universitas Gadjah Mada

Infectious Myonecrosis (IMN), which is caused by Infectious Myonecrosis Virus (IMNV), is rerecognized as causative agents of serious systemic diseases that cause significant morbidity and mortality in cultured Litopenaeus vannamei. In 2006 the first disease outbreaks caused by IMNV in Asia occurred in Situbondo district,, Indonesian and with the mortality of more than 70%. Gene encode sub major capsid proteinof this virus was predicted has a specific segment gene that used to study the genetic variation due to random mutations in a spaces uniform and it has been shown to reflect evolutionary relationships and geographic origins also of IMN virus strains. The aims of this study were to determine the nucleotide and amino acid sequences in Indonesia and phylogenetic relation of gene encode IMN virus sub major capsid protein. Gene encode IMNV sub major capsid protein was amplified using one-step RT-PCR followed by nested PCR. Amplification process began from nucleotide 3841 to 4345 (#ABN05324.1 GenBank) in the coat protein segment. Sequencing and phylogenetic analysis were used to obtain the phylogenetic relation of IMN virus. Homology of nucleotide in the studied strains were 98,90% to 99.20% and comparison strains were 98.70% to 99.40%. Amino acid translation homology ranged from 98.30% to 99.10% and strain comparison by 98.30% to 99.70%. Cladogram analysis constructed by neighbor-Joining and maximum parsimony algorithm showed that 3 isolates IMNV from (Lampung, Pontianak, and Banyuwangi) all IMNV 100% valid one group / family, samples from Lampung IMNV Banyuwangi identical to the original, a cluster with a strain comparison IMNV from Indonesia from GenBank (47%) and Pontianak IMNV different with 2 other sample origin (Lampung, and Banyuwangi) despite low validity (60%). IMNV-Brazil showed distinct phylogenetic relationship of the isolates of cluster-Indonesia.strain IMNV research closely related virus strains IMNV_Brazil and IMNV_Indonesia (100).