DETEKSI KETERBAWAAN VIRUS PADA BENIH KACANG PANJANG
Seed health is one of the main factors on long bean�s (Vigna sinensis L.) cultivation, so seed quality should have important role on the production. BCMV (Bean common mosaic virus) is an important disease that transmitted by seed. Besides BCMV on the long bean, nowadays yellow disease has been...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/120158/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=60178 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Seed health is one of the main factors on long bean�s (Vigna sinensis L.)
cultivation, so seed quality should have important role on the production. BCMV (Bean
common mosaic virus) is an important disease that transmitted by seed. Besides BCMV
on the long bean, nowadays yellow disease has been spread widely and very harm, so
the preventif actions is needed. One of the preventif actions of virus disease is through
healthy seed. The aims of this research were to know the health of commercial long
bean seed, the occurence of virus on seed from diseased plant, and the type of virus
which infected long bean. The methods used on this research were by visual observation
of seed, growing-on test, molecular detection, protein analysis and observation of virus
morphology using electron microscope. Seed cultivars used in this research were New
Jaliteng, Java Plus, Mahkota 01, Hijau Super, Brigade and collection seed from Plant
Virology Laboratory of Gadjah Mada University. The result revealed that seed from a
pack of commercial seed showed malformation and didn�t have uniformity in size. The
collection seed from plant with yellow symptoms showed malformation and wrinkled
symptoms. The result on growing-on test method showed leaves with mosaic,
yellowing, stunted, curly and malformation symptoms, but molecular detection and
protein analysis of BCMV and Begomovirus didn�t show the DNA band or protein band
which indicated virus attack. Long bean seed which was tested and showed symptoms
on growing-on test revealed that it didn�t bring neither BCMV or Begomovirus. This
research also proved that Begomovirus on long bean wasn�t transmitted by produced
seed. |
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