ANALISIS GENETIK MOLEKULER CACING Ascaridia galli DI YOGYAKARTA BERDASARKAN SEKUEN RIBOSOMAL DNA (rDNA) REGIO ITS1

• Main Authors: , DYAH UTAMI, , drh. Ana Sahara, M.Si.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/121062/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61109
• Institution: Universitas Gadjah Mada

Ascaridia galli is an endoparasite of intestinal that important enough to poultry. Genetic analysis in needed to know the diversity. Composition and length of ITS1 is vary so it can be used for study about diversity. The main purpose of this research is to know the sequen of ribosomal DNA regio ITS1 so that and the genetic relationship between some species of Ascaridia galli can be analyzed. The DNA sample was isolated from male Ascaridia galli that has identificated. The extraction of DNA was done with a kit Geneaid®. Amplification of ITS1 gene by PCR technique used primer AG_1F and AG_1R under condition of predenaturation 94oC for 5 minutes, denaturation 94oC for 30 seconds, annealing 55oC for 45 seconds, elongation 72oC for 45 seconds, dan post-elongation 72oC for 5 minutes at 30 cycles. The PCR reaction produced 655 bp DNA segment which has followed by sequencing. Then result of ITS1 segment sequences would be treated in multiple allignment with other worms from Ordo Ascaridida that were taken from the Genbank and then analyzed using MEGA version 5.01. Result of this research showed that there were 6 nt different beetwen sample of Ascaridia galli and Ascaridia galli isolate China (AM408550.1) with the genetic distance is 0,018 (1,8%). It showed that the sample of Ascaridia galli has a close genetic relationship with Ascaridia galli isolate China. Construction of phylogenetic trees used Neighbor-Joining method with bootstrap values 1000 times showed that the classification made by the morfological sign was the same with the classification made by ribosomal DNA regio ITS1 sequence.