ISOLASI DAN IDENTIFIKASI BAKTERI PADA SUSU MASTITIS SUBKLINIS DI KOPERASI TERNAK SARONO MAKMUR SABRANG WETAN WUKIRSARI CANGKRINGAN SLEMAN
Subclinical mastitis was one of causes of decreasing milk production. The various species of bacteria could be the cause of subclinical mastitis. This research was aim to isolate and identify the bacteria in subclinical mastitis milk from Sarono Makmur farm group in Sabrang Wetan Wukirsari Cangkring...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/121084/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61136 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Subclinical mastitis was one of causes of decreasing milk production. The
various species of bacteria could be the cause of subclinical mastitis. This
research was aim to isolate and identify the bacteria in subclinical mastitis milk
from Sarono Makmur farm group in Sabrang Wetan Wukirsari Cangkringan
Sleman Jogjakarta.
Twenty (20) milk samples from Sarono Makmur farm group were use in
this research. Milk samples tested with California Mastitis Test (CMT) test for
determine the subclinical mastitis and get 16 samples were positive subclinical
mastitis and 4 samples were negative. The 16 subclinical mastitis milk samples
then do isolation on selective media and biochemical test with planting on some
media such as sugars (mannitol, glucose, lactose, sucrose, maltose), catalase test,
coagulase test, oxidase, motility test, simone citrate test, IMViC test, urease test,
and DNAse test.
The results of bacterial isolation from 16 samples subclinical mastitis
were found Escherichia coli 11 isolate (68.75%), Bacillus cereus 5 isolate
(31.25%), Diplococcus sp 2 isolate (12.5%), Staphylococcus aureus 2 isolate
(12.5%), Klebsiella pneumonia, 2 isolate (12.5%), Stomacoccus sp 2 isolate
(12.5%), Klebsiella ozaena 1 isolate (6.25%), Staphylococcus epidermidis 1
isolate (6.25%) and Micrococcus sp 1 isolate (6.25%). Escherichia coli found in
high percentage (68.75%) indicate the hygiene management of the dairy cow was
worse. Improved sanitation, milking procedures, maintenance management,
improvement expected to suppress the growth of pathogenic bacteria causing
subclinical mastitis in dairy were needed. |
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