PENGARUH SUPLEMENTASI FETAL CALF SERUM TERHADAP KEMAMPUAN MATURASI IN VITRO OOSIT SAPI

• Main Authors: , Denvy Meidian Daoed, , Ir. Diah Tri Widayati, MP., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/121582/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61676
• Institution: Universitas Gadjah Mada

This researcs was conducted to utilize the ovaries from the Slaughterhouse (RPH) as oocytes source for in vitro fertilization (IVF). To increase the successfull of IVF, FCS were supplementation into in vitro maturation medium. From the slaughterhouse ovaries were transported to the laboratory in 31-34ºC, 0.9% NaCl. Furthermore, oocytes were aspirated by using a 3 ml syringe and 23 G needle containing Dulbeco�s-Phosphate Buffer Saline (DPBS), then maturated in modified CO2 incubator (99% humidity and a temperature of 39ºC) in medium Tissue Culture Medium-199 (TCM-199). Oocytes were divided into 2 groups: control group (TCM-199) and the treatment group (TCM-199 + 10% FCS). Data was analyzed by Chi-Square, while the quality of oocytes were analyzed descriptively. Maturation ability of bovine oocytes in the treatment group was significantly higher than the control group (127 ± 22.35 (55.22%) vs 89 ± 16.33 (40.09%). Finaly, suplementation of 10% FCS in maturation medium improve the quality and ability of oocytes maturation.