PENGARUH KONSENTRASI Cu-ZEOLIT DALAM AKUADES TERHADAP PERTUMBUHAN Streptococcus mutans
Streptococcus mutans is one of many microorganisms composing denture plaque. Its development can be prevented by using denture cleanser (soaking method). Denture cleanser can be produced by combination of zeolite and copper (Cu) through ion exchange, thus producing mixture of antibacterial. Study ai...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/121904/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62003 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Streptococcus mutans is one of many microorganisms composing denture
plaque. Its development can be prevented by using denture cleanser (soaking
method). Denture cleanser can be produced by combination of zeolite and copper
(Cu) through ion exchange, thus producing mixture of antibacterial. Study aims to
understand the effect of Cu-zeolite concentration in the distilled water affecting
Streptococcus mutans growth.
The study used material of natural zeolite (Wonosari), CuCl2. 2H2O
(Merck p.a, Germany), and cultured S. mutans. Zeolite was activated by
increasing temperature for 1 hour (105oC) and mixed with 0.2 M CuCl2 solution
in 50g:250mL ratio using stir heat for 1 hour (60°C). Solution washed and dried
within 24 hours (100oC). Cu-zeolite was dilluted according to each concentration
of 9mg/mL, 18mg/mL and 36mg/mL. Its contacted with 0,01mL suspension of S.
mutans (108 CFU/mL) for 120 minutes and implanted on BHI agar, and incubated
(37oC/24 hours). Number of colonies counted and analyzed using Anava test and
LSD0,05.
Mean and standard deviation of the number of colonies on Cu-zeolite
concentration in the aquades of 9mg/mL, 18mg/mL and 36mg/mL were 40,000
±14,142, 22,500 ±5,000, and 10,000 ±0 (CFU/mL), respectively. Anava test
indicates Cu-zeolite concentration in aquades decreasing the growth of S. mutans
colonies with a significant difference in the concentration groups of 9mg/mL and
36mg/mL. The result it can be concluded that the Cu-zeolite solution decreased
the growth of S. mutans. High concentration proven to be effective on decreasing
numbers of S.mutans. |
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