PENGARUH PEMBERIAN BUBUK TULANG AYAM SEBAGAI SUPLEMEN SELAMA MASA KEHAMILAN DAN MENYUSUI TERHADAP JUMLAH AMELOBLAS DAN KETEBALAN EMAIL GIGI ANAK

• Main Authors: , ANGGI ARLAN, , Drg. Alma Linggar Jonarta, M. Kes.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/122085/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62184
• Institution: Universitas Gadjah Mada

Nutrition is one of the important factor for ameloblast and enamel on tooth development. Chicken bone powder containing various proteins and minerals that can be used as alternative nutrition suplement, has good bioavailibility. The aim of this research was to examined the effects of chicken bone powder supplementation during pregnancy and lactation on ameloblast and enamel of Sprague dawley�s rat pups. Nine female pregnant rats were devided into 2 group: treatment and control group (comparator and negative control subgroup). Chicken bone powder was administered to female rats starting from day 0 of pregnancy period to day 5 of lactation period. A suspension of 13,75% chicken bone powder was given by intragastric administration once a day. Obimin AF® (1,5%) or CMC-Na 2,5% was used as comparator or negative control subgroup, respectivelly. The suspension of each group was given 2 ml once a day. On day 5 afterbirth, four pups of each mother were sacrificed and the mandibula was removed, processed, and embedded in paraffin. Five μm of tissues were cut and stained with Haematoxylineosin, continued by histomorphometric analysis of ameloblast number and enamel thickness. The picture of ameloblast and enamel were taken under light microscope by the magnification of 100 or 1000x, consecutively. The pictures were further analysed using computer software image analysis �KLONK�. Statistical analysis was done by using ANOVA. The number of ameloblast and enamel thickness of pups from mother treated with chicken bone powder were significantly better than negative or comparator control subgroup (p�0,05). This results indicated that the supplementation of chicken bone powder during pregnancy and lactation period might improve the number of ameloblast and enamel thickness during tooth development.