PERBANDINGAN JUMLAH SEL DAN INTI SEL RAKSASA TIPE BENDA ASING PASCAIMPLANTASI HIDROKSIAPATIT DAN KARBONAT APATIT (Kajian in vivo pada Subkutan Tikus)

• Main Authors: , GLORIA FORTUNA, , dr. Dyah Listyarifah, M.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/122246/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62345
• Institution: Universitas Gadjah Mada

Hydroxyapatite (HA) has a good bioactivity and osteoconductivity, but it is difficult to be resorbed by the body. Carbonated hydroxyapatite (CHA) has the same advantages with HA, but it is easier to be resorbed by the body. Implantation of synthetic material initiates foreign body reaction, which is characterized by foreign body giant cells (FBGCs) formation. Soft tissue biocompatibility test is required prior clinical application of bone substitute since the material contact with the soft tissue upon application and can cause severe inflammatory response. The aim of this study is to evaluate the numbers of FBGC and their nuclei after implantation of HA and CHA. Hydroxyapatite and CHA were implanted in subcutaneous tissue of the back of 12 male Rattus norvegicus. Each 3 rats were terminated after 7, 14, 21, 28 days of implantation. The implantation area was taken for histological preparations and stained with Haematoxylin-Eosin. The number of FBGCs and their nuclei were observed at 6 fields of view by 2 observers using trinocular light microscope with magnification of 400x. The data of the number of FBGC was analyzed with Two-way ANOVA (p<0.05). The data of the number of FBGC nuclei was analyzed with nonparametric ANOVA test. It showed that the number of FBGC between HA and CHA after 14, 21, 28 days of implantation were significantly different. There were no significant difference in the number of FBGC nuclei between HA and CHA implantation. This study showed that HA leads higher number of FBGC than CHA on subchronic phase.