ISOLASI DAN IDENTIFIKASI BAKTERI Aeromonas sp. DARI LELE DUMBO (Clarias sp.) DI KABUPATEN NGAWI

• Main Authors: , SRI REJEKI, , Dr. Ir. Murwantoko, M.Si.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/122366/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62468
• Institution: Universitas Gadjah Mada

This objective of the research was to study isolation, identification and pathogenicity Aeromonas bacteria on African catfish (Clarias sp.). The samples of African catfish have been obtained from three sub district in Ngawi regency. There were 15 isolates obtained were injected (0,1 ml/fish) to African catfish based on Koch postulates test, external and internal symptomps of African catfish were observed. Characterization and identification through morphology of bacterial colonies and cells, physiological and biochemical test. Pathogenicity test was done by injected with concentrate of 104, 106, 108 cfu/ml in 7-9 cm catfish size. Bacterial pathogenecity based on value of Lethal Dosage (LD50). The result showed 12 isolates (GKJ1, GKJ3, GKJ4, GGN1, GGN2, GGN3, GGN4, GGN5, GGN6, GPR2, GPR3 and GPR4) were identified as Aeromonas hydrophila with percentage values of 96,67%, 93,33%, 93,33%, 90%, 90%, 90%, 86,67%, 96,67%, 86,67%, 93,33%, 90% and 90%. The disease symptoms which attacted by the bacteria generally were skin ulcer, abdominal swelling and kidney damage. Isolate of A. hydrophila GKJ1, GKJ4, GGN2, GGN5, GPR2 and GPR4 were virulent on African catfish with LD50 values of (1,55 x 106), (3,89 x 106), (7,24 x 106), (2,39 x 106), (6,61 x 105) and (1,95 x 105 cfu/ml). Three isolates (GKJ2, GKJ5 and GPR1) were identified as A. salmonicida with percentage values of 86,67%, 83,33% and 83,33% .