LATIHAN FISIK TERATUR DAN TERUKUR PADA TIKUS Sprague Dawley PASCAOVARIEKTOMI DALAM MENCEGAH RISIKO ARITMIA VENTRIKULER

• Main Authors: , Denny Agustiningsih, dr.,M.Kes., , Prof.dr. Sri Kadarsih S, MSc, Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/122442/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62544
• Institution: Universitas Gadjah Mada

Background: Ventricular arrhythmia therapy in postmenopausal women using antiarrhythmic aggravate the risk of arrhythmia, whereas the use of estrogen replacement therapy is still controversial. Ventricular arrhythmias can prevented with regular and measurable physical exercise because the effect on heart rate, action potential duration, repolarization duration, expression of Ca and K ion channels in cardiac ventricular muscle. Regular and measurable physical exercise also stimulate extragonadal synthesis of estrogen. Regularly and measurable physical exercise of the ovariectomized rats needs to be proven prevent ventricular arrhythmias through electrical activity changes and estrogen synthesis in cardiac ventricular muscle. Method: The subjects are Sprague Dawley rats aged 4-5 weeks were ovariectomized and given regularly and measurable physical exercise using a treadmill (Gamatread version 2006) for 12 weeks at an intensity equivalent to 60-70% VO2max achieved by graded exercise. Determination of the QTc interval using a special formula for rats (Kmecova & Klimas). Expression of Ca and K ion channels, CYP19aromatase, COX2 and estrogen receptor α in cardiac muscle using IHC. To determine the effects of exercise on Ca and K ion channel using diphenylalkylamin and amiodarone. Statistical analysis using paired t test, ANOVA and correlation analysis. Results: The ovariectomized and exercise group that given regular and measurable physical exercise for 12 weeks lead to QTc interval prolongation (61.58+4.84 to 69.11+2.63 ms) following the RR interval prolongation (125.63+6.23 to 142.19+7,49ms) and a decrease in heart rate (471.69 +24.49 to 414.24 +24.36 x/min) and is correlated (r=0,358) with serum estrogen levels (16.64+5.42 to 18.40+6.3 pg/dL). Ovariectomized with exercise group have a Ca ion channel expression (85.64 +3.46%) which is higher than group without exercise (67.26 +2.33%), as well as the expression of K ion channels 48.12 +0.55% compared to 39.36 +3.00%, both closely related to QTc interval (r=0,508 and r=0,517). Ovariectomized with exercise group has CYP19aromatase expression (44.43 +5.56%) which is higher than group without exercise (34.09 +2.62%), as well as estrogen receptor α 47.80+1.86 % compared to 42.29 +4.56%. Conclusion: Regular and measurable physical exercise of postovariectomized rats prevent ventricular arrhythmias by causing prolongation of the QTc interval 7,8% through the increase expressions of Ca and K ion channels. Regular and measurable physical exercise of postovariectomized rats lead to cardiac muscle cells estrogen synthesis through the increase of COX2 and CYP19aromatase expression Regular and measurable physical exercise of postovariectomized rats lead to increase the expression of estrogen receptor α in the heart muscle.