Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD
Study on diversity among eight Dendrobium spp. orchids is necessary for orchid breeders in order to plant crossing efficiency. Highly similarity among Dendrobium spp. would increase crossing opportunity. The objectives of this study were to grouping orchid genotypes into clusters according to their...
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[Yogyakarta] : Universitas Gadjah Mada
2013
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id-ugm-repo.1225042016-03-04T08:30:12Z https://repository.ugm.ac.id/122504/ Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD , DHARMESTA ADHIWIRA , Dr. Ir. Aziz Purwantoro, M.Sc. ETD Study on diversity among eight Dendrobium spp. orchids is necessary for orchid breeders in order to plant crossing efficiency. Highly similarity among Dendrobium spp. would increase crossing opportunity. The objectives of this study were to grouping orchid genotypes into clusters according to their similarity as measured by DNA banding patterns. Genetic similarity among 10 orchid genotypes were analysed based on 85 bands generated from five RAPD primers. The result showed that genetic diversity within species indicated by percentage of polymorphic loci was in range of 11.76 to 40%. Cluster analysis based on molecular markers showed that the Dendrobiums were divided into six groups at a distance of 7.5 in dendogram, where is first group consisted of D. spectabile and D. macrophyllum, second group consisted of D. lasianthera, third group consisted of D. antennatum, D. discolor, and D. stratiotes, fourth group consisted of D. smillieae and D. crumenatum, fifth group consisted of D. signatum, and sixth group consisted of D. fimbriatum. Two Dendrobium spesies from another country (D. fimbriatum and D. signatum) made a group outside of Dendrobium of Indonesia�s group, indicating that both spesies have distant genetic similarity with Dendrobium spesies of Indonesia. Result of grouping based on molecular markers is different from grouping based on morphological observation, probably due to environment effects. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , DHARMESTA ADHIWIRA and , Dr. Ir. Aziz Purwantoro, M.Sc. (2013) Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62608 |
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ETD , DHARMESTA ADHIWIRA , Dr. Ir. Aziz Purwantoro, M.Sc. Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
description |
Study on diversity among eight Dendrobium spp. orchids is necessary for orchid
breeders in order to plant crossing efficiency. Highly similarity among Dendrobium spp.
would increase crossing opportunity. The objectives of this study were to grouping
orchid genotypes into clusters according to their similarity as measured by DNA
banding patterns. Genetic similarity among 10 orchid genotypes were analysed based on
85 bands generated from five RAPD primers. The result showed that genetic diversity
within species indicated by percentage of polymorphic loci was in range of 11.76 to
40%. Cluster analysis based on molecular markers showed that the Dendrobiums were
divided into six groups at a distance of 7.5 in dendogram, where is first group consisted
of D. spectabile and D. macrophyllum, second group consisted of D. lasianthera, third
group consisted of D. antennatum, D. discolor, and D. stratiotes, fourth group consisted
of D. smillieae and D. crumenatum, fifth group consisted of D. signatum, and sixth
group consisted of D. fimbriatum. Two Dendrobium spesies from another country (D.
fimbriatum and D. signatum) made a group outside of Dendrobium of Indonesia�s
group, indicating that both spesies have distant genetic similarity with Dendrobium
spesies of Indonesia. Result of grouping based on molecular markers is different from
grouping based on morphological observation, probably due to environment effects. |
format |
Theses and Dissertations NonPeerReviewed |
author |
, DHARMESTA ADHIWIRA , Dr. Ir. Aziz Purwantoro, M.Sc. |
author_facet |
, DHARMESTA ADHIWIRA , Dr. Ir. Aziz Purwantoro, M.Sc. |
author_sort |
, DHARMESTA ADHIWIRA |
title |
Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
title_short |
Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
title_full |
Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
title_fullStr |
Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
title_full_unstemmed |
Keragaman Delapan Anggrek Dendrobium spp. Indonesia Berdasarkan Penanda RAPD |
title_sort |
keragaman delapan anggrek dendrobium spp. indonesia berdasarkan penanda rapd |
publisher |
[Yogyakarta] : Universitas Gadjah Mada |
publishDate |
2013 |
url |
https://repository.ugm.ac.id/122504/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62608 |
_version_ |
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