ISOLASI Amylomyces rouxii DARI RAGI DAN TAPE SINKONG SERTA PRODUKSI ENZIM GLUKOAMILASE
Glucoamylase is one of starch degrading enzyme that can be used in food and non-food industry. Glucoamylase can be produced by mold, such as Amylomyces rouxii. A. rouxii has natural habitat on fermentated tape and can be isolated directly from tape starters or casava tape. The objetives of this rese...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Online Access: | https://repository.ugm.ac.id/122611/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62717 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Glucoamylase is one of starch degrading enzyme that can be used in
food and non-food industry. Glucoamylase can be produced by mold, such
as Amylomyces rouxii. A. rouxii has natural habitat on fermentated tape and
can be isolated directly from tape starters or casava tape. The objetives of
this research is to obtain A. rouxii isolate from tape starters and cassava tape
found in Yogyakarta that produced high glucomyamylase activity. This
study was also carried out to evaluate conditions for glucoamylase
production from the A. rouxii.
Isolation step was done using 2 kinds of tape starter and cassava tape
from local market of Yogyakarta. The samples were inoculated in potato
dextrose agar medium by the spread plate method. Screening of amylolitik
activity from the isolates suspect were done by measuring iodine clear zone
that surrounding the isolates. Microscopic morphology of the isolates were
also observe. Production of glucoamylase were carried out in various
medium containg basal salts and sekam, bekatul, or kethak as a carbon
source. The enzyme activity was measured using soluble starch as a
substrate and the reducing sugar was monitored by the method of
cuppromonium complex.
The results show that 6 strains of fungi were isolated from the
samples. From these, three isolates showed the similar morphology with
Amylomyces rouxii. A.rouxii B had highest glucoamylase activity. The best
growth conditions of A.rouxii for producing glucoamylase enzyme were
using sekam medium, and incubation at 37°C for 3 days. At these condition,
the enzyme produced was 104.45 U/ gram medium. |
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