PENGARUH CARA PENGOLAHAN DAN JUMLAH DAUN SIRIH MERAH (Piper crocatum) TERHADAP PERTUMBUHAN Porphyromonas gingivalis (Kajian in vitro)

• Main Authors: , FEBTIAN KHUSNANTI, , drg. Nunuk Purwanti, M.Kes., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/122713/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62817
• Institution: Universitas Gadjah Mada

Porphyromonas gingivalis is one of the bacteria that cause periodontal disease. Red betel leaf contains antibacterial compounds. There are two processing method of red betel leaf�s in the community that the efectivity hasn�t been proven yet. The aim of this study is to determine the inhibition of Porphyromonas gingivalis bacteria after application of 10 and 20 pieces of red betel leaves that boiling and steeping with aquades. Porphyromonas gingivalis was used a pure culture with the consentration of 108CFU/ml. The effect of 10 and 20 pieces boiling water and steeping water of red betel leaf (Piper crocatum) to the growth of Porphyromonas gingivalis was determined by measuring the optical density (OD) of Porphyromonas gingivalis using spectrophotometre with 600nm wavelength. The initial OD was measured before incubation, while the latter OD was measured after incubation for 24 hours. The final OD was obtained from the latter OD minus the initial OD. This study showed that the application of boiling water and steeping water of red betel leaf (Piper crocatum) with the number of 10 and 20 pieces does not give a significantly effect to the growth of Porphyromonas gingivalis. This result was probably caused by the thickness of the membrane structure of Porphyromonas gingivalis bacteria and the mechanism of protection, acted by the outer membrane and periplasmic and caused by also the lack of active antibacterial compounds of red betel leaf (Piper crocatum) with 10 and 20 pieces of leaves.