PENGARUH PERLAKUAN EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L) DAN SUHU PENYIMPANAN TERHADAP PERUBAHAN KADAR HISTAMIN, TMA-N DAN TVB-N IKAN TUNA (Thunnus sp)

• Main Authors: , LISA S.P. DAVIDZ, , Dr. Ir. Suparmo, M.Sc
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123262/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63373
• Institution: Universitas Gadjah Mada

Jatropha curcas leaf is traditionally used, in Moluccas region, to minimize the off flavor and itchy consequence after consumption of slightly off quality tuna fish (Thunus sp). The study was conducted to evaluate the solvent extraction of Jatropha curcas leaves, soaking time of tuna meat in Jatropha extract, and storage temperature of the treated tuna meat on changes in levels of histamine, TMA-N and TVB-N of the fresh tuna fish. Solvents used in the leaf extraction were water, key lime, citric acid (4%, 8%, and 12%), ratio of fish meat with the leaf extract, and the storage temperatures (10±1o C, 20±1o C, 30±2o C and 37±1o C) with seven different sampling times of each storage temperatures. Based on evaluation on the quality changes of the fish, soaking time of 4 minutes was chosen in accordance to those used traditionally. The utilization of key lime and cittric acid of 12% showed the lowest changes in histamine, TMA-N, and TVB-N contents during storage at several temperatures, and only storage at 30oC showed the best effect of the leaf extract utilization. The length of the soaking time did not show any effect on TVB-N and TMA-N. The ratio of amount of leaf and solvent during extraction affect penetration of the extract into the fish tissue and bacterial infestation, as appear in the low increase of TMA-N and TVB-N. The higher the storage temperature increase TMA-N faster but not the case with Histamine changes. Histamine changes was clearly aggravated by the increase of bacterial growth.