ISOLASI, IDENTIFIKASI BAKTERI DARI OVARIUM YANG MENGALAMI INFEKSI (OOFORITIS) PADA AYAM PETELUR KOMERSIAL DAN UJI SENSITIVITAS TERHADAP BEBERAPA JENIS ANTIBIOTIK

• Main Authors: , NOVIANTI NELIYANI TOELLE, , Dr. drh. Agnesia Endang Tri Hastuti Wahyuni, M.Si
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123459/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63570
• Institution: Universitas Gadjah Mada

Ovary infection (oophoritis) is among the most common diseases found in layers between the start of egg production and during the laying phase. The incidence of oophoritis may entend into oviduct to cause salphyngitis or vice versa. Generally, control of oophoritis was done with antibiotics, however antibiotic treatment was frequently improper that can support the emergence of bacterial resistance. Oophoritis cases in Indonesia has not been widely reported, however the incidence of decreased egg production often occurred. Oophoritis causing bacteria and antibiotic usage for treatment is also unknown well. Therefore, this experiment was designed to isolate and to identify the bacteria causing ovary infection in commercial layers and to evaluate bacterial sensitivity to some antibiotics that commonly used in the field. Nineteen samples of commercial layers that were obtained from three farms in the region of Yogyakarta were necropsied. Samples of infected ovaries were cultured on blood agar plates (PAD), then incubated at 370C for 18-24 hours in aerobic and anaerobic. Gram staining and biochemical tests were performed to identify the bacteria. After bacteria have been identified, sensitivity test was performed by disc diffusion method of Kirby-Bauer. Bacteria that can be isolated and identified from infected ovary were Staphylococcus aureus (29%), Escherichia coli (21%), Staphylococcus intermedius (17%), Staphylococcus epidermidis (13%), Streptococcus sp. (4%), Bacillus sp. (4%), Bacillus cereus (4%), Enterobacter sp. (4%), and Klebsiella pneumonia (4%). Antibiotic sensitivity testing showed that bacteria originated from infected ovary were sensitive to the combination of amoxylin-clavulinic acid (88%), gentamicin (88%), enrofloxacin (71%), and erythromycin (58%). Based on these results, it can be concluded that the most common bacteria that can be isolated from infected ovary were Staphylococcus aureus and Escherichia coli. Most of the bacteria isolated from infected ovary were sensitive to the combination of of amoxylin-clavulinic acid, gentamicin, enrofloxacin, and erythromycin, however they were resistant to colistin sulfate, tetracycline, and penicillin.