EKSPRESI SIKLOOKSIGENASE-2 DAN INFILTRASI SEL INFLAMASI GINGIVA PADA TIKUS YANG DIINDUKSI PERIODONTITIS SETELAH PEMBERIAN EKSTRAK ETANOLIK KULIT MANGGIS
Periodontitis is a chronic inflammatory periodontal tissues which caused by periodontopathic bacteria. Clinically, this disease characterized by the redness of gingival margin, loss of gingival attachment and alveolar bone resorption. COX-2 is an enzyme which plays an important role in inflammation....
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/123589/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63702 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Periodontitis is a chronic inflammatory periodontal tissues which caused by
periodontopathic bacteria. Clinically, this disease characterized by the redness of
gingival margin, loss of gingival attachment and alveolar bone resorption. COX-2
is an enzyme which plays an important role in inflammation. The level of this
enzyme increases in periodontitis cases. Mangosteen rind has xantone, an antiinflammatory
subtance which inhibits the synthesis of PGE2 through inhibition of
COX-2 secretion. This research aimed to study the expression of COX-2 and
gingival inflammatory cells infiltration in experimental-induced periodontitis in
rats after mangosteen rind etanolic extract administration.
Forty-eight male wistar rats were used in this study. The experimental
periodontitis model was induced by silk ligature, which was placed subgingivally
around the neck of the anterior lower teeth for 7 days. After the ligation was taken
out, the rats were divided into 4 treatment groups, namely mangosteen rind extract
30 mg/kg, 60 mg/kg, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd,
5th and 7th day after the treatment. The anterior lower jaws were then processed for
paraffin embedded tissue. Hematoxillin Eosin staining was done to examine the
number of inflammatory cells infiltration, while immunohistochemistry using
antibody anti-COX-2 were done to recognized the COX-2 expression. The
number of inflammatory cells was analyzed by Two Way Anova test and COX-2
expression by Kruskall Wallis test.
Anova result showed a significant difference among groups (p<0.05)
indicating that mangosteen rind etanolic extract affected the number of
inflammatory cells infiltration. Kruskall Wallis showed a significant difference
among groups (p<0.05) indicating that mangosteen rind etanolic extract affected
the expression of COX-2. In conclusion, mangosteen rind etanolic extract reduced
gingival inflammatory cells infiltration and COX-2 expression in experimentalinduced
periodontitis in rats |
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