ANALISIS PROFIL ALELIK ISOLAT MULTIDRUG- RESISTANT Streptococcus pneumoniae PADA SALURAN NASOFARING PASIEN TERINFEKSI HIV DI JAKARTA MENGGUNAKAN MULTILOCUS SEQUENCE TYPING

• Main Authors: , TIARA PUTRI, , Prof. Drs. Langkah Sembiring, M.Sc., Ph.D.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2013
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/123603/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63716
• Institution: Universitas Gadjah Mada

Multidrug-resistant S. pneumoniae isolates in HIV infected patients were studied to determine their allelic profile. Samples were collected from the nasopharyngeal tract of HIV infected children and adults. Nasopharyngeal swabs were then cultured on blood agar medium and series of biochemical, microbiological, and molecular tests were performed to identify S. pneumoniae. Results showed that serotypes 19F, 6A/B, and 19A were predominant in children, while serotypes 6A/B, 19F, and 11A were predominant in adults. Antibiotic susceptibility test using disk diffusion method revealed that all isolates were sensitive to amoxicillin and cefotaxime, but some of them were resistant to cefixime and sulphamethoxazole-trimethoprim. Fourteen multidrug-resistant isolates were subsequently assessed using multilocus sequence typing and 7 allelic profiles were identified. Most prevalent allelic profiles were ST320 and ST271. Similar resistance pattern to clindamycin and sulphamethoxazole-trimethoprim was found in group ST320. Multidrug-Resistant isolates which were nonsusceptible to the same antibiotics shared the same allelic profile, or were at least different at only one single locus. Phylogenetic tree construction of multidrug-resistant isolates based on concatenated sequences of housekeeping genes showed that isolates grouped according to their allelic profiles. It can be concluded that there was a congruence between allelic profiles and clade groups in the phylogenetic tree. Moreover, similar resistance pattern to clindamycin and sulphamethoxazoletrimethoprim found in group ST320 can give suggestions for more appropriate antibiotic treatment in the future.